Nano-Structured Hydrogels for Macromolecular Separations

9521381洛克这个项目正在研究开发和表征新的纳米结构多孔介质，以改进通过电泳法或层析法分离生物大分子。通过使用形成溶致液晶相的半刚性棒状聚离子或两亲性(表面活性剂)来模版内部结构，将产生具有限定直径在大约2到几纳米范围内的定制内部通道或孔的水凝胶。将制备各向同性和各向异性沟道化(因此纳米结构)介质。这项研究的具体目标包括：(1)合成几类纳米结构水凝胶；(2)开发和应用表征水凝胶内部孔结构的新方法；(3)测量结构化和常规水凝胶中分子的尺寸排斥极限和传输特性(电泳和扩散)；以及(4)模拟分子在常规和结构化凝胶中的传输。***

9521381 Locke This project is on research to develop and characterize new, nano-structured porous media for improved separations of biological macromolecules by electrophoresis or chromatography. Hydrogels with tailored internal channels or pores having defined diameters in the range from approximately 2 to several nanometers will be created by using semi-rigid rod-like polyions or amphiphiles (surfactants) that form lyotropic liquid crystalline phases to template the internal structure. Both isotropic and anisotropic channelized (hence nano-structured) media will be prepared. The specific aims of this research include: (1) synthesis of several classes of nano-structured hydrogels; (2) development and application of new methods for characterizing the internal pore structures of hydrogels; (3) measurement of size exclusion limits and transport properties (electrophoretic and diffusive) of molecules within structured and conventional hydrogels; and (4) modeling molecular transport in conventional and structured gels. ***