Stopped-Flow Spectrophotometer for Kinetic Investigation and Time Resolved Optical Spectra of Reactive Intermediates

这份来自化学研究仪器和设施项目的合同将帮助加州大学圣巴巴拉分校化学系获得一台应用光物理停流动力学分光光度计。利用这些仪器进行的研究项目包括：铁血红蛋白的还原性亚硝化，与酶促溴过氧化物酶活性有关的钒(V)过氧化物物种的机制，以及活性硝基芳烃和氮杂环反应中的中间体的表征。停流动力学分光光度计在研究非常快的化学反应时是有用的。止流法允许在不到1毫秒的时间内混合两种溶液。这提供了一个完整的浓度-时间记录的反应物溶液。在研究酶催化反应和通常需要快速混合以进行动力学研究的催化时，止流法特别有用。

This award from the Chemistry Research Instrumentation and Facilities Program will assist the Department of Chemistry at the University of California-Santa Barbara to acquire an Applied Photophysics stopped-flow kinetics spectrophotometer. Research projects to be carried out with these instruments include: reductive nitrosation of ferriheme proteins, mechanisms of vanadium(V) peroxo species relevant to the enzymatic bromoperoxidase activity, and the characterization of intermediates in reactions of activated nitroarenes and nitrogen heterocycles. The stopped-flow kinetics spectrophotometer is useful when studying very fast chemical reactions. The stopped-flow method permits mixing of two solutions in less than one (1) millisecond. This provides a complete concentration-time record of the reactant solutions. The stopped-flow method is especially useful when studying enzyme-catalyzed reactions and in catalysis where the common need is rapid mixing for kinetic studies.