Stopped-Flow Spectrophotometer for Kinetic Investigation and Time Resolved Optical Spectra of Reactive Intermediates

化学研究仪器和设施项目的这一奖项将帮助加州大学圣巴巴拉分校化学系获得一台应用光物理学停流动力学分光光度计。将利用这些仪器进行的研究项目包括：铁血红素蛋白的还原亚硝化，钒(V)过氧物与酶促溴过氧化物酶活性相关的机理，以及活化的硝基芳烃和氮杂环反应中中间体的表征。停流动力学分光光度计在研究非常快的化学反应时很有用。停流法允许在不到一(1)毫秒的时间内混合两种溶液。这提供了反应物溶液的完整浓度-时间记录。停流法在研究酶催化反应时特别有用，在催化中通常需要快速混合以进行动力学研究。

This award from the Chemistry Research Instrumentation and Facilities Program will assist the Department of Chemistry at the University of California-Santa Barbara to acquire an Applied Photophysics stopped-flow kinetics spectrophotometer. Research projects to be carried out with these instruments include: reductive nitrosation of ferriheme proteins, mechanisms of vanadium(V) peroxo species relevant to the enzymatic bromoperoxidase activity, and the characterization of intermediates in reactions of activated nitroarenes and nitrogen heterocycles. The stopped-flow kinetics spectrophotometer is useful when studying very fast chemical reactions. The stopped-flow method permits mixing of two solutions in less than one (1) millisecond. This provides a complete concentration-time record of the reactant solutions. The stopped-flow method is especially useful when studying enzyme-catalyzed reactions and in catalysis where the common need is rapid mixing for kinetic studies.