Stopped-Flow Spectrophotometer for Kinetic Investigation and Time Resolved Optical Spectra of Reactive Intermediates

用于反应中间体的动力学研究和时间分辨光谱的停流分光光度计

• 批准号: 9522259
• 负责人: Peter Ford
• 金额: $ 5.19万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-12-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9522259&HistoricalAwards=false
• 关键词: Stopped; Flow; Spectrophotometer; Kinetic; Investigation

This award from the Chemistry Research Instrumentation and Facilities Program will assist the Department of Chemistry at the University of California-Santa Barbara to acquire an Applied Photophysics stopped-flow kinetics spectrophotometer. Research projects to be carried out with these instruments include: reductive nitrosation of ferriheme proteins, mechanisms of vanadium(V) peroxo species relevant to the enzymatic bromoperoxidase activity, and the characterization of intermediates in reactions of activated nitroarenes and nitrogen heterocycles. The stopped-flow kinetics spectrophotometer is useful when studying very fast chemical reactions. The stopped-flow method permits mixing of two solutions in less than one (1) millisecond. This provides a complete concentration-time record of the reactant solutions. The stopped-flow method is especially useful when studying enzyme-catalyzed reactions and in catalysis where the common need is rapid mixing for kinetic studies.

化学研究仪器和设施计划的这一奖项将有助于 加州大学圣巴巴拉分校化学系获得应用 光物理停流动力学分光光度计。 将与这些仪器进行的研究项目包括：亚铁血红素蛋白质的还原亚硝化，钒（V）过氧物种相关的酶溴过氧化物酶活性的机制，以及活性硝基芳烃和氮杂环反应中间体的表征。 停流动力学分光光度计在研究非常快速的化学反应时非常有用。 停流法允许两种溶液在不到一（1）毫秒内混合。 这提供了反应物溶液的完整浓度-时间记录。 停流法在研究酶催化反应和催化反应时特别有用，其中通常需要快速混合以进行动力学研究。