SGER: Plasmapheresis After Partial Hepatectomy or Nephrectomy

当一个肾脏或部分肾脏被手术切除或功能失调时，剩余肾脏的质量以代偿性方式增加，以维持正常或接近正常的肾功能。调节这种代偿性变化的过程尚不清楚。在这个提议中，辛普森博士提出了一个假设，即代偿性生长是血液中通常抑制组织生长的器官特异性因子受到抑制的结果。因此，当这个因子被抑制时，特定的组织开始增殖。生长的典型特征是细胞分裂率的增加。他认为，抑制物质是由器官（在这种情况下是肾脏）自身产生的，以防止过度生长。切除或失去一个器官会降低血浆中抑制剂的浓度，从而允许细胞生长。他将通过将一只正常动物的血浆注入一只已经切除三分之二肾脏的动物体内来验证这一假设。这应该可以防止细胞生长。在输注供体血浆24小时后，通过DNA合成（加入3h -胸腺嘧啶）跟踪细胞生长（分裂）。这些实验有可能为正常和异常细胞生长和调控提供有价值的见解。＊＊＊

9522534 Simpson When one kidney or part of a kidney is surgically removed or becomes dysfunctional, the mass of the remaining kidney increases in a compensatory manner to maintain normal or near normal renal function. The processes that regulate the compensatory changes are not understood. In this proposal, Dr. Simpson puts forth the hypothesis that compensatory growth is the result of the inhibition of a organ-specific factor in the blood that normally suppresses tissue growth. Thus, when this factor is inhibited, specific tissues begin to proliferate. The growth is typified by an increased rate of cell division. He suggests that the suppressant material is produced by the organ (kidney in this case) itself to prevent over growth. Removal or loss of an organ reduces the plasma concentration of the suppressant and consequently permits cell growth. He will test this hypothesis by infusing plasma from a normal animal into one where two thirds of the kidney has been removed. This should prevent cell growth. Cell growth (division) will be tracked by DNA synthesis (incorporation of 3H-thymidine) 24 hours after the infusion of donor plasma. These experiments have the potential of providing valuable insights into normal and abnormal cell growth an regulation. ***