Biomolecular Engineering Studies of Extremely Thermophilic Xylose-Glucose Isomerases

极嗜热木糖-葡萄糖异构酶的生物分子工程研究

• 批准号: 9529047
• 负责人: J. Gregory Zeikus
• 金额: $ 24.05万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-15 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9529047&HistoricalAwards=false
• 关键词: Biomolecular; Engineering; Studies; Extremely; Thermophilic

9529047 Zeikus This project is on research to study aspects of biocatalysis and thermostability for extremely thermophlic xylose (glucose) isomerases (GIs). The research will focus on GIs from the thermophilic and the hyperthermophilic eubacterial genera Thermoanaerobacterium and Thermotoga, and will attempt to identify the structural bases for thermal stability and differing catalytic activities for this family of enzymes. In addition, these enzymes will be model systems for studying substrate binding and the role of metal ions in the enzymes structure and function. The sequences and crystal structures of these enzymes will be compared to the less thermostable GIs. ***

9529047 Zeikus该项目是关于研究极端嗜热木糖（葡萄糖）异构酶（GI）的生物催化和热稳定性方面的研究。 这项研究将集中在地理标志从嗜热和超嗜热真细菌属Thermoanaerobacterium和Thermotoga，并将试图确定热稳定性和不同的催化活性，这个家庭的酶的结构基础。 此外，这些酶将是研究底物结合和金属离子在酶结构和功能中的作用的模型系统。 这些酶的序列和晶体结构将与较不耐热的GI进行比较。 ***