Biomolecular Engineering Studies of Extremely Thermophilic Xylose-Glucose Isomerases

本课题主要研究极耐热性木糖（葡萄糖）异构酶（gi）的生物催化和热稳定性。本研究将重点关注嗜热性和超嗜热性真细菌属热厌氧细菌和热菌属的gi，并将尝试确定该家族酶的热稳定性和不同催化活性的结构基础。此外，这些酶将成为研究底物结合和金属离子在酶结构和功能中的作用的模型系统。这些酶的序列和晶体结构将与耐热性较差的酶进行比较。＊＊＊

9529047 Zeikus This project is on research to study aspects of biocatalysis and thermostability for extremely thermophlic xylose (glucose) isomerases (GIs). The research will focus on GIs from the thermophilic and the hyperthermophilic eubacterial genera Thermoanaerobacterium and Thermotoga, and will attempt to identify the structural bases for thermal stability and differing catalytic activities for this family of enzymes. In addition, these enzymes will be model systems for studying substrate binding and the role of metal ions in the enzymes structure and function. The sequences and crystal structures of these enzymes will be compared to the less thermostable GIs. ***