Collaborative Research: Bioprocessing and Engineering Studies of Extremely Thermophilic Xylose Isomerases

本项目的目标是继续密歇根州立大学和北卡罗来纳州州立大学之间的合作努力，探索II类木糖异构酶（XI）的嗜热性，最终目标是为上级异构化过程提供科学和工程基础。 具体的研究目标包括：（1）利用蛋白质工程开发一种上级Xi，该XI能够在高温和低pH下生产55%的果糖糖浆，不存在不需要的副产物形成，并且不需要色谱富集步骤;（2）解决与这种生物催化剂相关的生物加工问题;（3）研究II类XI之间的差异和相似性，作为开发对其他酶有用的热稳定和热活化策略的基础;（4）考虑在高温下操作并具有技术潜力的其他单糖生物转化。

The objective of this project is to continue collaborative efforts between Michigan State University and North Carolina State University to explore the thermophilicity of class II xylose isomerases (XIs) with the ultimate goal of providing the scientific and engineering bases for a superior isomerization process. The specific research objectives include: (1) to use protein engineering to develop a superior XI able to produce a 55% fructose syrup at high temperature and low pH in the absence of unwanted by-product formation and without the requirement for a chromatographic enrichment step; (2) to address the bioprocessing issues relevant to such biocatalysts; (3) to examine the differences and similarities among class II XIs as a basis for developing thermostabilization and thermoactivation strategies useful for other enzymes; and (4) to consider other monosaccharide biotransformations that operate at elevated temperatures and that have technological potential.