SBIR Phase I: In-Situ Oceanographic Phytoplankton Sensor

SBIR 第一阶段：原位海洋浮游植物传感器

• 批准号: 9561010
• 负责人: Salvador Fernandez
• 金额: $ 7.5万
• 依托单位: CIENCIA INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-02-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9561010&HistoricalAwards=false
• 关键词: SBIR; Phase; Situ; Oceanographic; Phytoplankton

9561010 Fernandez This Small Business Innovation Research Phase I Project will develop a novel in situ fluorescence lifetime-based sensor for determination of small-scale phytoplankton distribution and physiological status. A method will be developed to determine phytoplankton physiological status from determination of cholorophyll a fluorescence lifetimes, and an inexpensive, rugged, compact instrument will be designed to implement the method in situ. Lifetime sensing will be performed using phase fluorometry. The instrument implementation is based on the use of a cross-correlation method using a modulated light-emitting diode (LED) as a light source and digital data acquisition an analysis. A resolution of the order of 10 ps will permit accurate determination of cholorophyll fluorescence lifetime changes that are know to occur under various physiological conditions. This may be the first oceanographic phytoplankton sensor to exploit fluorescence lifetime sensing. Potential commercial applications range from oceanographic sensing to laboratory research. Potential markets include plant nursery, breeding, tissue culture, plant genetic engineering, on -line process analysis in bioreactors, and aquaculture.

9561010费尔南德斯 这项小型企业创新研究第一期项目将开发一种新型的原位荧光寿命传感器，用于确定小型浮游植物的分布和生理状态。将开发一种方法来确定浮游植物的生理状态，从叶绿素a荧光寿命的测定，并将设计一个廉价的，坚固的，紧凑的仪器，以实现该方法在现场。将使用相位荧光测定法进行寿命感知。仪器的实现是基于使用的互相关方法，使用调制的发光二极管（LED）作为光源和数字数据采集分析。10 ps量级的分辨率将允许准确测定已知在各种生理条件下发生的叶绿素荧光寿命变化。 这可能是第一个利用荧光寿命传感的海洋浮游植物传感器。 潜在的商业应用范围从海洋传感到实验室研究。潜在的市场包括植物苗圃、育种、组织培养、植物基因工程、生物反应器中的在线过程分析和水产养殖。