Acquisition of an HPLC and GC-IRMS for Oceanographic, Biological and Environmental Engineering Research on Stable Isotope Composition of Individual Compounds

获取 HPLC 和 GC-IRMS，用于海洋学、生物和环境工程研究单个化合物的稳定同位素组成

• 批准号: 9601630
• 负责人: Susan Henrichs
• 金额: $ 16万
• 依托单位: University of Alaska Fairbanks Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9601630&HistoricalAwards=false
• 关键词: Acquisition; HPLC; GC; IRMS; Oceanographic

Funding is requested to purchase a gas chromatograph-isotope ratio mass spectrometer (GC-CIRMS). This instrument permits the measurement of carbon and nitrogen stable isotope ratios of a wide variety of organic molecules, essentially all of those which can be resolved by gas chromatography or which can be converted to a suitable volatile derivative. Also, funds are requested for an off-line, automated high-performance liquid chromatography (HPLC) system for isolation of specific compounds for subsequent, off-line, IRMS. This approach is needed for certain compounds which are too involatile or lack sufficient heat stability for GC; it also can be more convenient for compounds, such as amino acids, which require extensive derivatization for GC. Together, these instruments would constitute a facility that could measure the C and N isotopic composition of virtually any organic substance. Specifically, the HPLC and GC-IRMS would be used to measure the stable isotope composition of amino acids and fatty acids in the course of studies of the growth rate and trophic dynamics of marine mammals. The stable isotope composition of specific organic compounds, such as amino acids, carbohydrates, hydrocarbons, and lipids, from marine and lake sediments would be used in investigations of the sources and diagenetic alteration of these substances, assisting studies of environmental change at high northern latitudes. Also, the instruments would be used in tracer investigations of the metabolism of large mammals by measuring changes over time in the isotopic composition of metabolites such as volatile fatty acids. Finally, the GC-C-IRMS would be used to distinguish naturally-occurring hydrocarbons from petroleum-derived contaminants.

请求资金购买气相色谱同位素比质谱仪（GC-CIRMS）。该仪器可以测量各种有机分子的碳和氮稳定同位素比率，基本上所有可以通过气相色谱法解析或可以转化为合适的挥发性衍生物的分子。此外，还需要资金用于离线、自动化高效液相色谱 (HPLC) 系统，用于分离特定化合物以用于后续离线 IRMS。 对于某些太不挥发或缺乏足够的 GC 热稳定性的化合物需要这种方法； 它还可以更方便地分析氨基酸等需要进行大量GC衍生化的化合物。 这些仪器共同构成了一个可以测量几乎任何有机物质的 C 和 N 同位素组成的设施。 具体来说，在研究海洋哺乳动物的生长速率和营养动态过程中，HPLC和GC-IRMS将用于测量氨基酸和脂肪酸的稳定同位素组成。来自海洋和湖泊沉积物的特定有机化合物（例如氨基酸、碳水化合物、碳氢化合物和脂质）的稳定同位素组成将用于研究这些物质的来源和成岩作用，协助研究北高纬度地区的环境变化。此外，这些仪器还将用于通过测量挥发性脂肪酸等代谢物的同位素组成随时间的变化来对大型哺乳动物的新陈代谢进行示踪研究。最后，GC-C-IRMS 将用于区分天然存在的碳氢化合物和石油衍生的污染物。