Transcriptional Regulation of Potassium Current

钾电流的转录调节

基本信息

  • 批准号:
    9603978
  • 负责人:
  • 金额:
    $ 15万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    1997
  • 资助国家:
    美国
  • 起止时间:
    1997-03-15 至 2002-12-31
  • 项目状态:
    已结题

项目摘要

PI: Spitzer IBN: 9603978 Dr. Spitzer will analyze the molecular mechanisms that underlie expression of potassium current in embryonic spinal nerve cell. Nerve cells transmit information with electrical signals, and acquire the ability to generate these signals called action potentials at very early stages of differentiation. Initially, these action potentials allow the entry of substantial amounts of calcium ions. Dr. Spitzer has shown that this calcium influx generates important signals that regulate several aspects of the development of nerve cells. However, since this amount of calcium entry is toxic to mature nerve cells, the neurons must dramatically reduce the amount of calcium entry as they mature. In earlier work Dr. Spitzer showed that the reduction of calcium influx is the result of an increase in potassium current that flows out of the nerve cells through protein pores, called potassium channels, opposing the calcium influx. In the work supported by this grant he will determine the basis of the developmental increase in this potassium current. Preliminary results suggest that expression of a particular gene, known as Kv2.2, which encodes the protein of which potassium channels are composed, may be responsible for the increase in this potassium current. Dr. Spitzer will use antisense DNA sequences to suppress the synthesis of the protein in nerve cells isolated and growing in culture. He will take advantage of the availability of a toxin, hanatoxin, that is a specific blocker of these channels, to check the success of these experiments. If the antisense treatment suppresses expression of the Kv2.2 protein, the toxin will no longer affect the potassium current. The results from the project will provide new knowledge about the regulation of excitability in the spinal cord. The fact that the central nervous system of young vertebrates is especially vulnerable to epileptic seizure activity may be partly due to the calcium influx that is part of the early signaling mac hinery. In the future it may be possible to suppress this calcium influx selectively by regulating the expression of particular potassium channel genes.
PI:Spitzer IBN:9603978 Spitzer博士将分析胚胎脊髓神经细胞中钾电流表达的分子机制。 神经细胞通过电信号传递信息,并在分化的早期阶段获得产生这些称为动作电位的信号的能力。 最初,这些动作电位允许大量的钙离子进入。 斯皮策博士已经证明,这种钙离子的流入会产生重要的信号,调节神经细胞发育的几个方面。 然而,由于这种量的钙进入对成熟的神经细胞是有毒的,神经元必须在它们成熟时显著减少钙进入的量。 在早期的工作中,斯皮策博士表明,钙流入的减少是钾电流增加的结果,钾电流通过蛋白质孔(称为钾通道)流出神经细胞,与钙流入相反。 在这项资助支持的工作中,他将确定钾电流发展增加的基础。 初步结果表明,一种称为Kv2.2的特定基因的表达可能是导致钾电流增加的原因,该基因编码钾通道的蛋白质。 斯皮策博士将使用反义DNA序列来抑制分离的神经细胞中蛋白质的合成,并在培养中生长。 他将利用一种毒素的可用性,hanatoxin,这是一种特定的通道阻断剂,以检查这些实验的成功。 如果反义处理抑制Kv2.2蛋白的表达,则毒素将不再影响钾电流。 该项目的结果将提供有关脊髓兴奋性调节的新知识。 幼年脊椎动物的中枢神经系统特别容易受到癫痫发作活动的影响,这一事实可能部分是由于钙内流(早期信号机制的一部分)造成的。 在未来,可能通过调节特定钾通道基因的表达来选择性地抑制这种钙内流。

项目成果

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Nicholas Spitzer其他文献

Nicholas Spitzer的其他文献

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{{ truncateString('Nicholas Spitzer', 18)}}的其他基金

Role of postsynaptic transmitter receptors in influencing presynaptic neurotransmitter identity
突触后递质受体在影响突触前神经递质身份中的作用
  • 批准号:
    2051555
  • 财政年份:
    2022
  • 资助金额:
    $ 15万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Complex Dynamics of the Earthquake Recovery of an Ethnic Minority
博士论文研究:少数民族地震恢复的复杂动力学
  • 批准号:
    1323698
  • 财政年份:
    2013
  • 资助金额:
    $ 15万
  • 项目类别:
    Standard Grant
FASEB Summer Research Conference: Developmental Neurobiology; July 14-19, Saxton's River, Vermont
FASEB 夏季研究会议:发育神经生物学;
  • 批准号:
    8508521
  • 财政年份:
    1985
  • 资助金额:
    $ 15万
  • 项目类别:
    Standard Grant
Development of Reflex Behavior in a Simple Nervous System
简单神经系统反射行为的发展
  • 批准号:
    7923459
  • 财政年份:
    1980
  • 资助金额:
    $ 15万
  • 项目类别:
    Standard Grant
Anatomy and Physiology of Embryonic Sensory Neurons
胚胎感觉神经元的解剖学和生理学
  • 批准号:
    7608348
  • 财政年份:
    1976
  • 资助金额:
    $ 15万
  • 项目类别:
    Continuing Grant
Neural Integration and Geometry in the StomatogasTric Ganglion
口胃三叉神经节的神经整合和几何结构
  • 批准号:
    7301633
  • 财政年份:
    1973
  • 资助金额:
    $ 15万
  • 项目类别:
    Standard Grant

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