Gene Enzyme and Physiological Characterization of shrunken-2 Isoalleles

shrunken-2 等位基因的基因酶和生理特征

• 批准号: 9604612
• 负责人: L. Curtis Hannah
• 金额: $ 33万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 2001-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604612&HistoricalAwards=false
• 关键词: Gene; Enzyme; Physiological; Characterization; shrunken

9604612 Hannah Regulation of starch synthesis in maize kernels is the interest of Dr. Hannah for many years. The analysis of mutants in starch synthesis resulted in the detection of a likely rate-limiting step in the action and activity of the enzyme, ADP glucose pyrophosphorylase (AGP). The proposed work originates from the results of prior support in which regions (and amino acids) in the enzyme have been identified which determine its allosteric properties. In the continuation, Dr. Hannah will use a bacterial expression system for the production of enzymes generated by directed mutagenesis to obtain detailed knowledge about allosteric and catalytic domains of the enzyme, about the interaction of the two subunits of the enzyme, and screen for second-site suppressor mutants. He will then return to whole plant analysis by corn transformation of mutant enzymes into a background which contains a deletion of the gene. Dr. Hannah's work has been paradigmatic for the analysis of maize starch mutants and the extension of genetic analysis into biochemistry and plant physiology.

玉米籽粒中淀粉合成的调控是Hannah博士多年来的研究兴趣。对淀粉合成突变体的分析发现了ADP葡萄糖焦磷酸化酶（AGP）的作用和活性可能的限速步骤。提议的工作源于先前支持的结果，其中已经确定了酶中的区域（和氨基酸），这些区域决定了它的变构性质。在后续研究中，Hannah博士将使用细菌表达系统生产由定向诱变产生的酶，以获得关于酶的变构和催化结构域的详细知识，关于酶的两个亚基的相互作用，并筛选第二位点抑制突变体。然后，他将通过玉米将突变酶转化为包含基因缺失的背景进行全株分析。汉娜博士的工作是玉米淀粉突变体分析的典范，并将遗传分析扩展到生物化学和植物生理学。