Physiological role of PAFAH2, an oxidized phospholipid-hydrolyzing enzyme and PAFAH2-derived bioactive lipids

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In this study, we obtained following results:(1) Using immunoprecipitation coupled with mass spectrometry, several PAFAH2-interacting proteins including PRDX2 and SCP2 were identified.(2) From mutational analysis of PAFAH2, Cys-83 was found to be the only residue conferring sensitivity of PAFAH2 to DTNB, suggesting that PAFAH2 activity can be regulated by modification of Cys-83.(3) An immunocytochemical assay to detect the translocation of PAFAH2 from cytosol to membrane was established.(4) PAFAH2-deficient mice exhibited reduced PCA reaction most probably by impaired mast cell degranulation.(5) Bone marrow-derived mast cells from PAFAH2-deficient mice also showed decreased IgE-dependent degranulation.