Dedicated Stopped-Flow Spectrometer for CD, Absorbance and Fluorescence Measurements

用于 CD、吸光度和荧光测量的专用停流光谱仪

• 批准号: 9604752
• 负责人: Daniel Raleigh
• 金额: $ 3.38万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604752&HistoricalAwards=false
• 关键词: Dedicated; Stopped; Flow; Spectrometer; CD

Funds are requested for the acquisition of a stopped-flow spectrometer for absorbance, circular dichroism and fluorescence measurements. Projects will be conducted by six major users from the Departments of Physiology & Biophysics and Chemistry. The instrument will be used in investigations of protein folding, enzyme mechanisms, peptide and protein interactions with membranes. Prof. McLaughlin will study the interactions of the proteins Src and MARCKS with membranes. Prof. Miller will study the folding and assembly of multidomain proteins. Prof. Raleigh will use this instrumentation to help elucidate the folding pathway of two proteins. Prof. Sampson will study the mechanism of the important enzyme cholesterol oxidase. Prof. Scarlata will investigate the activation of phospholipase C by G proteins. Prof. Wishina will study the dynamics of molten globule states of proteins. The instrument will be housed in a shared instrumentation laboratory located in the Chemistry Department and operated as a user-accessible, operator maintained shared facility.

要求拨款购买一台止流光谱仪，用于吸光度、圆二色性和荧光测量。项目将由生理与生物物理系和化学系的六名主要用户进行。该仪器将用于研究蛋白质折叠、酶机制、肽和蛋白质与膜的相互作用。McLaughlin教授将研究Src和MARCKS蛋白与膜的相互作用。米勒教授将研究多结构域蛋白的折叠和组装。罗利教授将使用这种仪器来帮助阐明两种蛋白质的折叠途径。Sampson教授将研究重要的酶胆固醇氧化酶的作用机制。Scarlata教授将研究G蛋白对磷脂酶C的激活作用。Wishina教授将研究蛋白质的熔融球态动力学。该仪器将被安置在位于化学系的共享仪器实验室中，并作为用户可访问的、操作员维护的共享设施进行操作。