U.S.-Swedish Cooperative Research: Redox Titration of Protein Phosphorylation in Photosynthetic Membranes

国际和平协会和瑞典隆德大学的约翰·艾伦之间的这项国际合作有两个目标。一是进一步表征高等植物叶绿体、蓝藻类囊体膜和紫色细菌发色团的膜蛋白磷酸化的氧化还原控制。使用的主要方法是电位氧化还原滴定和磷成像。另一个是确定单独的磷蛋白在控制光能的收集和分配以及光合作用电子传递中的作用。在那里使用的主要方法是荧光光谱学，既可以探测单次电子转移，也可以探测稳态电子转移。隆德实验室将提供进行研究所需的设备和资源。合作的结果可能导致我们对光合作用基本过程的理解的突破，并可能应用于其他细胞系统。

This international collaboration between the PI and John Allen of Lund University, Sweden has two objectives. One is to further characterize redox control of the phosphorylation of membrane proteins from chloroplasts of higher plants, cyanobacterial thylakoid membranes, and chromatophores from purple bacteria. The primary method used will be potentiometric redox titration and phosphorimaging. The other is to determine the roles of individual phosphoproteins in controlling light-energy harvesting and distribution, as well as photosynthetic electron transfer. The main method used there will be fluorescence spectroscopy to probe both single turnover and steady-state electron transfer. The Lund laboratory will provide equipment and resources needed to perform the research. The result of the collaboration could lead to a breakthrough in our understanding of a process fundamental to photosynthesis, and could have application to other cell systems.