Plant Receptor-like Kinases in ROS Signaling (PROSIG)

ROS 信号转导中的植物受体样激酶 (PROSIG)

• 批准号: 105783258
• 负责人: Professorin Dr. Silke Robatzek
• 金额: --
• 依托单位: Institut für Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/105783258?language=en
• 关键词: Plant; Receptor; like; Kinases; ROS

Receptor-like/Pelle kinases (RLKs) are important components of many signaling networks in plants. RLKs control plant development and hormone responses, stomatal regulation, stress responses as well as defense against bacterial and fungal pathogens. The same RLK, like the ERECTA, BRI1 and SERK3 (BAK1), can function in development and in pathogen resistance or susceptibility to stress. The existence of transient and dynamic multireceptor signaling complexes consisting of main ligand-binding receptors and non-ligand-binding co-receptors can help to explain the observed multiple intersections between plant signaling pathways. The main objective of this project is therefore to determine how single RLKs discriminate diverse functions and supply specificity in stress signaling. To achieve this we aim to characterize multiple signaling networks involving RLKs involved in stress response and development in Arabidopsis and Barley by the integrated use of genomic, biochemical, molecular and genetic approaches. Investigating crossover between stress and developmental signaling is likely to yield new insights into the interactions the different pathways. Work will focus on selected members of the RLKs of group LRRII (total 14 members including the SERK family), group LRRXI (total 28 members including Clavata1), group LRRXII (total 10 members including FLS2 and EFR), the DUF26 group (total 35 members including the Cystein-rich kinases [CRK]) and the SLG (Self-incompatibility glycoprotein) group (total 31 members) with the following objectives:1) to determine the role of RLKs in ROS-mediated cellular and systemic signaling in biotic and abiotic stresses in Arabidopsis using existing and novel mutant collections and in barley using a novel antisense oligodeoxynucleotide technology, ODN (1) combined with genomic aproaches.2) to determine which RLKs involved in stress responses have a function in development3) analysis of multi-receptor signaling complex formation by competition FRET methodology, and development and optimization of FRET technology for in planta visualization of receptor interactions and activation. (proof of principle for construction of phosphorylation FRET biosensor).4) to identify the composition of the multiprotein RLK downstream signaling networks involved in ROS- and brassinosteroid-mediated signaling by genomic, biochemical and molecular biological approaches.

受体样/Pelle激酶(RLKs)是植物体内许多信号网络的重要组成部分。RLK控制植物的发育和激素反应、气孔调节、逆境反应以及对细菌和真菌病原体的防御。同样的RLK与ERECTA、BRI1和SERK3(BAK1)一样，可以在发育、病原菌抗性或对胁迫的敏感性中发挥作用。由主要配体结合受体和非配体结合辅助受体组成的瞬时和动态多受体信号复合体的存在有助于解释所观察到的植物信号通路之间的多重交叉。因此，该项目的主要目标是确定单个RLK如何区分不同的功能，并在应激信号中提供特异性。为了实现这一目标，我们的目标是通过综合使用基因组、生化、分子和遗传方法来描述涉及RLKs的多种信号网络，这些RLKs参与拟南芥和大麦的逆境反应和发育。研究压力和发育信号之间的交叉可能会对不同途径之间的相互作用产生新的见解。工作将集中在LRRII组(共14个成员，包括Serk家族)、LRRXI组(共28个成员，包括Clavata1)、LRRXII组(共10个成员，包括Fls2和EFR)、DUF26组(共35个成员，包括富含半胱氨酸激酶[CRK])和自交不亲和糖蛋白组(共31个成员)中的选定成员，目的如下：1)利用现有的和新的突变体库，确定RLK在ROS介导的细胞和系统信号转导的生物和非生物胁迫中的作用ODN(1)结合基因组途径2)确定哪些RLK参与应激反应在发育中起作用3)用竞争FRET方法分析多受体信号复合体的形成，以及FRET技术的发展和优化，以实现受体相互作用和激活的平面可视化。(构建磷酸化FRET生物传感器的原则证明)。4)通过基因组、生化和分子生物学方法，鉴定参与ROS和油菜素类固醇介导的信号转导的多蛋白RLK下游信号网络的组成。