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Receptor-like kinase palmitoylation: resolving a crucial feature of plant cell signalling

受体样激酶棕榈酰化：解决植物细胞信号传导的一个关键特征

基本信息

批准号：
BB/M024911/1
负责人：
Piers Hemsley
金额：
$ 42.52万
依托单位：
University of Dundee
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2015
资助国家：
英国
起止时间：
2015 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FM024911%2F1
关键词：
Receptor like kinase palmitoylation resolving

项目摘要

Regulating plant perception - the role of protein palmitoylationPlants are unable to move away from danger and must therefore be able to detect any potential threat or dangerous change in the environment and adapt accordingly to survive. Plants are also multicellular organisms with complex body plans composed of many specialized tissue and cell types. Organising and maintaining their structure requires tightly regulated communication between the individual cells making up the plant. Many of these developmental and environmental signals are detected by a group of proteins called receptor-like kinases (RLKs). RLKs are the largest group of proteins in plants, accounting for 2.5% of all the genes in the model organism Arabidopsis thaliana. RLKs detect signals from outside of the cell such as pathogens, hormones, changes in the integrity and structure of the cell wall or patterning and developmental signals produced by the plant itself. These signals are transmitted across the cell membrane to start intracellular responses such as altering gene activity, changing cell division and differentiation, modifying the cell wall or production of chemicals to fight off pathogens. Understanding how RLK signaling is regulated is therefore critical to all aspects of plant biology.Each RLK detects a different signal and in response to signals bind to specific protein partners to start the appropriate signaling processes. When RLKs interact they alter each other through a process known as post-translational modification. Post-translational modification usually involves the addition or removal of small molecules, such as phosphate groups, to specific amino acids in a proteins structure to alter its activity, change which proteins it can interact with, promote its degradation or change where in the cell the protein is found. Two post-translational modifications of RLKs are fairly well understood; phosphorylation and ubiquitination. Phosphorylation of RLKs occurs after signal perception and interaction of the RLK with its signaling partner, and generally causes activation of signaling. Ubiquitination usually results in the RLK being degraded to stop further signaling. I have recently discovered an entirely novel post-translation modification of RLKs that is essential for their correct signaling. This modification is called palmitoylation. I have found that unstimulated RLKs are palmitoylated but when they detect a relevant signal their palmitoylation state rapidly decreases. We currently don't know why RLKs are de-palmitoylated or when it happens in relation to other events in RLK signaling. This proposal seeks to answer these questions and characterize the role of this novel and important factor controlling plants primary method of responding to extracellular signals. We will also identify the enzymes required to de-palmitoylate RLKs to understand how this process is regulated.This greater knowledge of RLK function will help us to understand how plants regulate responses to threats, environmental problems and how they regulate their development. This information can be used in crop breeding and development programs to introduce or improve desired traits in plants and provide more insight into how plants will respond to particular circumstances. Interestingly plant RLKs are related to the Toll-like and interleukin receptors used by mammalian immune systems to detect pathogens, activate and enhance immunity and regulate inflammation. I have recently shown that these receptors are regulated in the same way as plant RLKs meaning that the information from my plant work can be able to help understand immune system regulation in humans.

调节植物感知——蛋白质棕榈酰化的作用植物无法摆脱危险，因此必须能够检测到环境中的任何潜在威胁或危险变化，并相应地适应才能生存。植物也是多细胞生物体，具有由许多专门的组织和细胞类型组成的复杂的身体结构。组织和维持其结构需要严格调节构成植物的各个细胞之间的通讯。许多发育和环境信号是由一组称为受体样激酶 (RLK) 的蛋白质检测到的。 RLK 是植物中最大的蛋白质组，占模式生物拟南芥所有基因的 2.5%。 RLK 检测来自细胞外部的信号，例如病原体、激素、细胞壁完整性和结构的变化或植物本身产生的图案和发育信号。这些信号穿过细胞膜传输，启动细胞内反应，例如改变基因活性、改变细胞分裂和分化、修改细胞壁或产生化学物质来抵抗病原体。因此，了解 RLK 信号传导如何调节对于植物生物学的各个方面都至关重要。每个 RLK 都会检测到不同的信号，并响应信号与特定的蛋白质伙伴结合以启动适当的信号传导过程。当 RLK 相互作用时，它们通过称为翻译后修饰的过程相互改变。翻译后修饰通常涉及在蛋白质结构中的特定氨基酸上添加或去除小分子（例如磷酸基团），以改变其活性、改变可以与之相互作用的蛋白质、促进其降解或改变蛋白质在细胞中的存在位置。 RLK 的两种翻译后修饰已相当容易理解；磷酸化和泛素化。 RLK 的磷酸化发生在信号感知以及 RLK 与其信号传导伴侣相互作用之后，通常会导致信号传导激活。泛素化通常会导致 RLK 降解，从而停止进一步的信号传导。我最近发现了一种全新的 RLK 翻译后修饰，这对于它们的正确信号传导至关重要。这种修饰称为棕榈酰化。我发现未受刺激的 RLK 会被棕榈酰化，但当它们检测到相关信号时，它们的棕榈酰化状态会迅速降低。我们目前不知道为什么 RLK 会去棕榈酰化，也不知道它何时发生与 RLK 信号传导中的其他事件相关。该提案旨在回答这些问题，并描述这种控制植物响应细胞外信号的主要方法的新颖且重要的因素的作用。我们还将鉴定 RLK 去棕榈酰化所需的酶，以了解该过程是如何调节的。对 RLK 功能的更多了解将有助于我们了解植物如何调节对威胁、环境问题的反应以及它们如何调节其发育。这些信息可用于作物育种和开发计划，以引入或改善植物所需的性状，并更深入地了解植物如何应对特定环境。有趣的是，植物 RLK 与哺乳动物免疫系统用来检测病原体、激活和增强免疫力以及调节炎症的 Toll 样受体和白细胞介素受体相关。我最近证明这些受体的调节方式与植物 RLK 相同，这意味着我的植物工作中的信息可以帮助了解人类免疫系统的调节。