The contribution of SERK family receptors to immunity against Xylella fastidiosa

SERK家族受体对木霉免疫的贡献

• 批准号: 402386942
• 负责人: Professorin Dr. Silke Robatzek
• 金额: --
• 依托单位: Institut für Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/402386942?language=en
• 关键词: contribution; SERK; family; receptors; immunity

Plant diseases represent a significant threat to global food security. One of the pathogens ranked in the “list of priority pests” for Europe is Xylella fastidiosa. This insect-transmitted bacterium is the cause of the OLIVE QUICK DECLINE SYNDROME (OQDS) and important for outbreaks over the past eight years. A sustainable strategy to manage OQDS is to breed disease resistance into susceptible hosts. However, only few resistant olive cultivars have been identified and classical breeding approaches will be long-term, taking little advantage of the molecular knowledge on plant immune mechanisms. This project will evaluate the processes that control the infection of X. fastidiosa in susceptible and resistant olive. Having established X. fastidiosa infection in the genetic model A. thaliana, we identified that BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE (BAK1) controls X. fastidiosa infection success. BAK1 belongs to the family of leucine-rich repeat-receptor-like kinases called SOMATIC EMBRYOGENESIS RECEPTOR KINASEs (SERK). BAK1/SERK3 forms complexes with pattern recognition receptors (PRR) such as the EF-Tu RECEPTOR (EFR) to induce PRR-triggered immunity (PTI). Transcriptome profiling in olive revealed that most OeSERK3 homologues were downregulated in the susceptible “Ogliarola salentina” cultivar. In resistant “Leccino”, more OeSERKs were upregulated in response to X. fastidiosa infection, and we speculate that some could function as co-receptors with PRRs. We also established callus and cell suspension cultures of both resistant and susceptible olive cultivars, which we explore for characterizing prototypic PTI responses as well as the generation of transgenic olives, i.e. transformed with EFR. Our goal is to answer two key questions: 1. How do OeSERKs regulate PTI and the response to X. fastidiosa infection? Profiling OeSERKs from olive and comparing cultivated varieties will reveal candidate genes subjected to functional analysis. OeSERK interacting proteins will be explored across the two cultivars. 2. What are the PTI signatures associated with olive immunity and Xylella’s infection success? Comparative transcriptome profiling of a resistant and susceptible olive cultivar together with assessing prototypic PTI responses to a panel of immune triggers in the olive family are likely to provide insights into this question. Being a woody perennial and non-model plant, our overall aim is to better understand PTI in olive and explore the cultivar and family genetic diversity for PTI profiling and the identification of molecular components associated with immunity. At its completion, this project will lead to new insights into the olive immune system and identify OeSERKs and interactors involved in the response to X. fastidiosa. Understanding immune co-receptor diversity is essential to engineer resistance into susceptible crop genotypes.

植物病害是对全球粮食安全的重大威胁。在欧洲的“优先害虫清单”中排名的病原体之一是苛养木杆菌。这种昆虫传播的细菌是橄榄快速衰退综合征（OQDS）的原因，并且在过去八年中对爆发很重要。管理OQDS的可持续策略是将抗病性培育到易感宿主中。然而，只有少数抗橄榄品种已被确定和经典的育种方法将是长期的，利用植物免疫机制的分子知识很少。该项目将评估控制X感染的过程。Fastidiosa在感病和抗病橄榄中的分布。建立了X.在遗传模型A. thaliana中，我们鉴定了油菜甾醇不敏感1-相关激酶（BAK 1）控制X。苛养菌感染成功。BAK 1属于称为体细胞胚胎发生受体激酶（SERK）的富含亮氨酸重复受体样激酶家族。BAK 1/SERK 3与模式识别受体（PRR）如EF-Tu受体（EFR）形成复合物以诱导PRR触发的免疫（PTI）。橄榄中的转录组分析显示，大多数OeSERK 3同源物在易感的“Ogliarola salentina”品种中下调。在抗性“Leccino”中，更多的OeSERKs响应于X而上调。fastidiosa感染，我们推测，一些可以作为共受体与PRRs。我们还建立了抗性和易感橄榄品种的愈伤组织和细胞悬浮培养物，我们探索其表征原型PTI反应以及转基因橄榄的产生，即用EFR转化。我们的目标是回答两个关键问题：1。OeSERKs如何调节PTI和对X.苛养菌感染？从橄榄中分析OeSERKs并比较栽培品种将揭示进行功能分析的候选基因。OeSERK相互作用蛋白将在两个品种中进行探索。2.与橄榄免疫和Xylella感染成功相关的PTI特征是什么？比较转录组分析的抗性和敏感的橄榄品种一起评估原型PTI反应的一组免疫触发器在橄榄家族可能提供深入了解这个问题。作为一种木本多年生和非模式植物，我们的总体目标是更好地了解橄榄PTI，并探索品种和家庭的遗传多样性PTI谱和鉴定与免疫相关的分子组分。在完成后，该项目将导致对橄榄免疫系统的新见解，并确定OeSERKs和参与对X反应的相互作用者。苛养。了解免疫共受体多样性对于将抗性工程化到易感作物基因型中至关重要。