Purchase of Instrumentation for Peptide Synthesis and Purification

购买肽合成和纯化仪器

• 批准号: 9614050
• 负责人: John Birks
• 金额: $ 5.5万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9614050&HistoricalAwards=false
• 关键词: Purchase; Instrumentation; Peptide; Synthesis; Purification

This award from the Chemistry Research Instrumentation and Facilities Program will assist the Department of Chemistry at the University of Colorado at Boulder to acquire an automated solid- phase peptide synthesizer and a high performance liquid chromatograph (HPLC). This equipment will enhance research in a number of areas including the following: (1) studies utilizing peptides to probe intermolecular tuning of protein signaling, (2) studies of the structural ramifications of glycosylation on peptide conformation, (3) synthesis and characterization of phosphopeptide analogues, (4) structural characterization of the protein folding intermediates and protein design, and (5) studies of the MAP kinase signal transduction pathway. The peptide synthesizer and the high performance liquid chromatograph (HPLC) are used by biological scientists to make and purify analogs of the natural substrates/effectors of a variety of proteins.

这项来自化学研究仪器和设施计划的奖项将协助位于博尔德的科罗拉多大学化学系获得自动固相肽合成仪和高效液相色谱仪（HPLC）。 这些设备将加强若干领域的研究，包括：（1）利用肽来探测蛋白质信号传导的分子间调节的研究，（2）糖基化对肽构象的结构分支的研究，（3）磷酸肽类似物的合成和表征，（4）蛋白质折叠中间体的结构表征和蛋白质设计，（5）MAP激酶信号转导通路的研究。 肽合成仪和高效液相色谱仪（HPLC）被生物科学家用于制备和纯化各种蛋白质的天然底物/效应物的类似物。