Purchase of Instrumentation for Peptide Synthesis and Purification

化学研究仪器和设施计划的这一奖项将帮助科罗拉多大学博尔德分校的化学系获得一台自动固相多肽合成器和一台高效液相色谱仪(HPLC)。该设备将加强以下领域的研究：(1)利用多肽探索蛋白质信号的分子间调谐；(2)糖基化对多肽构象的结构影响的研究；(3)磷酸多肽类似物的合成和表征；(4)蛋白质折叠中间体的结构表征和蛋白质设计；以及(5)MAP激酶信号转导途径的研究。生物科学家使用多肽合成器和高效液相色谱仪来制造和纯化各种蛋白质的天然底物/效应物的类似物。

This award from the Chemistry Research Instrumentation and Facilities Program will assist the Department of Chemistry at the University of Colorado at Boulder to acquire an automated solid- phase peptide synthesizer and a high performance liquid chromatograph (HPLC). This equipment will enhance research in a number of areas including the following: (1) studies utilizing peptides to probe intermolecular tuning of protein signaling, (2) studies of the structural ramifications of glycosylation on peptide conformation, (3) synthesis and characterization of phosphopeptide analogues, (4) structural characterization of the protein folding intermediates and protein design, and (5) studies of the MAP kinase signal transduction pathway. The peptide synthesizer and the high performance liquid chromatograph (HPLC) are used by biological scientists to make and purify analogs of the natural substrates/effectors of a variety of proteins.