Purchase of Instrumentation for Peptide Synthesis and Purification

购买肽合成和纯化仪器

• 批准号: 9614050
• 负责人: John Birks
• 金额: $ 5.5万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9614050&HistoricalAwards=false
• 关键词: Purchase; Instrumentation; Peptide; Synthesis; Purification

This award from the Chemistry Research Instrumentation and Facilities Program will assist the Department of Chemistry at the University of Colorado at Boulder to acquire an automated solid- phase peptide synthesizer and a high performance liquid chromatograph (HPLC). This equipment will enhance research in a number of areas including the following: (1) studies utilizing peptides to probe intermolecular tuning of protein signaling, (2) studies of the structural ramifications of glycosylation on peptide conformation, (3) synthesis and characterization of phosphopeptide analogues, (4) structural characterization of the protein folding intermediates and protein design, and (5) studies of the MAP kinase signal transduction pathway. The peptide synthesizer and the high performance liquid chromatograph (HPLC) are used by biological scientists to make and purify analogs of the natural substrates/effectors of a variety of proteins.

化学研究仪器和设施计划的奖项将协助科罗拉多大学博尔德分校的化学系获得自动化的固相肽合成器和高性能液相色谱仪（HPLC）。 该设备将在许多领域的研究中加强研究：（1）利用肽探测蛋白质信号传导的分子间调节的研究，（2）研究糖基化的结构性分析在肽构象上的结构分析，（3）（3）磷酸化肽类似物的合成和表征（4）蛋白质折叠（4）蛋白质折叠（4）蛋白质折叠（4）蛋白质折叠的蛋白质差异（4）激酶信号转导途径。 生物科学家使用肽合成器和高性能液相色谱仪（HPLC）来制作和净化各种蛋白质的天然底物/效应子的类似物。