Hybrid Magnetic Field Gradient Enhanced Colloidal Separations

提案编号： CTS-9618635提案类型： SGER主要研究者： Arijit Bose附属机构： 罗得岛大学 研究人员提议使用磁场将功能化磁性颗粒移动通过新型基于亲和力的分离器。 工作的目的是使这些功能化的磁性颗粒与靶分子（或细胞）接触，而不会产生显著的持续性。 该研究的新奇在于涉及可调磁场的反应器设计，允许在径向和/或轴向方向上的磁梯度。 使用功能化的磁性/超顺磁性颗粒来去除特定的分子/细胞等是至关重要的。 例如，如果癌细胞可以从骨髓中去除，患者自己的骨髓可以重新植入（化疗后），从而消除了对移植的需要。 有几家小型创业公司已经成功地制造了功能化的磁性颗粒，PI将使用其中一家（Bioquest）的颗粒。 但是简单地使用机械搅拌使细胞与功能化颗粒接触导致剪切诱导的细胞死亡。 因此，PI正在解决的问题是将扩散和对流传输的化学工程基本原理应用于分离器设计中的新问题的理想情况。 新颖之处在于问题和分离器设计。 如果成功的话，该方案可以通过使用连续系统允许更大的吞吐量并且另外维持细胞活力来彻底改变细胞分离领域。 这确实是一个高回报的项目。 同样令人兴奋的是，分离器原型可以用SGER的最小投资建造。

Proposal No: CTS-9618635 Proposal Type: SGER Principal Investigator: Arijit Bose Affiliation: University of Rhode Island The investigator proposes to use magnetic fields to move functionalized magnetic particles through a novel affinity based separator. The objective of the work is to allow these functionalized magnetic particles to contact target molecules (or cells) without the significant sustained. The novelty of the research is the reactor design involving tunable magnetic fields allowing magnetic gradients in the radial and /or axial directions. The use of functionalized magnetic/superparamagnetic particles to remove specific molecules/cells etc. is of vital importance. For example, if cancerous cells can be removed from bone marrow, the patient's own bone marrow can be re-implanted (after chemotherpy) thereby eliminating the need for a transplant. There are several small startup companies that have succeeded in making functionalized magnetic particles and the PI will use the particles from one of them (Bioquest). But simply using mechanical agitation to contact cells with the functionalized particles leads to shear induced cell death. Hence the problem the PI is attacking is an ideal case of applying chemical engineering fundamentals of diffusion and convective transport to a novel problem in separator design. The novelty is the problem and the separator design . If successful, this scheme could revolutionize the field of cell separations, by allowing greater throughput by using a continuous system, and additionally sustaining cell viability. This is truly a high payoff project. What is also exciting is that the seperator prototype can be built with the minimal investment of the SGER.