REG: Digital Imaging and Analysis for Electron Microscopy in Nanotechnology

摘要：Arijit BoseU.罗德岛大学将购买一台数码相机、接口设备和图像分析软件，为JEOL 1200 EX扫描/透射电子显微镜(S/透射电子显微镜)提供直接数字成像能力。该设备最初将用于一系列研究项目，这些项目代表了URI化学工程、生物医学科学、机械工程和物理系以及杜兰大学化学工程系之间的跨学科合作。这些包括：(A)阳离子/阴离子表面活性剂混合体系中微观结构演变的时间分辨冷冻-电子显微镜实验，(B)脂/肽组合形态的检查，模型膜蛋白在脂双层和膨胀胶束中的特定位置的测定(使用低温-电子显微镜成像)，从而揭示其功能；(C)微观结构的测定(低温-电子显微镜在新发现的水凝胶中具有几乎相等的水和有机相)，(D)扫描电子显微镜对由带有空心微球填充物的聚合物制成的新型复合材料的断口进行研究，以及(E)磁性纳米颗粒的形态、尺寸分布和聚集特征。数字成像和分析将极大地提高电子显微镜的使用效率和质量，并产生远远超出所述具体项目的关键影响。它将使电子显微镜成为大学几个系的学生的强大教育工具，并使他们能够获得胶体、材料科学和生物观察等关键技术方面的专业知识。

ABSTRACTCTS-0079330Arijit BoseU. of Rhode IslandThe University of Rhode Island will purchase a digital camera, interface equipment and image analysis software to provide direct digital imagining capability for the JEOL 1200 EX Scanning/Transmission Electron Microscope (S/TEM). The equipment will be used initially for a series of research projects representing interdisciplinary collaborations between Is in the Chemical Engineering, Biomedical Sciences, Mechanical Engineering and Physics departments at URI as well as the Chemical Engineering Department at Tulane University. These include (a) time-solved cryo-TEM experiments on microstructure evolution in mixed cationic/anionic surfactant systems, (b) examination of morphologies of lipid/peptide assemblies, determination of specific locations of model membrane proteins in lipid bilayers and swollen micelles (imaged using cryo-TEM) that will expose their functionalities, (c) determination of microstructures (cryo-TEM in newly discovered hydrogels that have nearly equal amounts of water and organic phase (d) SEM examination of fracture surfaces of a new class of composite materials made from polymers with cenosphere fillers, and (e) morphology, size distribution and aggregation characteristics of magnetic nanoparticles (TEM). Digital imaging and analysis will dramatically increase the efficiency and quality of utilization of the electron microscope, and have a critical impact far beyond the specific projects outlined. It will make the electron microscope available as a powerful educational tool for students from several departments in the university, and allow then to gain expertise in a vital technique for colloid, materials science and biological observations.