Instrumentation for Large Scale Neuron Population Recording

大规模神经元群记录仪器

• 批准号: 9619063
• 负责人: Donald Woodward
• 金额: $ 60万
• 依托单位: Wake Forest University School of Medicine
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9619063&HistoricalAwards=false
• 关键词: Instrumentation; Large; Scale; Neuron; Population

The overall goal of this instrument development for Systems Neurophysiology is to create functional devices to enable recording from very large ensembles of neuronal spike trains in behaving animals. The plan is to develop and integrate three tasks to be done in parallel. First, a new fabrication process will be employed to create thin film silicon recording probes, each with sixteen sites. The probe shapes and surface recording site properties will be optimized for long-term chronic recording to match the effectiveness of microwire arrays previously perfected by the P.I. Different spacing and sizes of surface recording sites will be patterned on the probe and different recording surfaces will be tested. A novel technology for fabrication of flexible microcables and multichip modules will be adapted to lead signals from the recording probes to external signal processing electronics. A second task is to create a design for a VLSI microchip to detect and amplify- signals from 16 channels. and multiplex signals to a single output lead. The chip will also provide switches with control to apply test/stimulation to a selected input. Later goals will be to produce a multisite probe with integrated active electronics and to create multiple probe assemblies. The processes to be developed are exceptionally favorable for commercial production methods. A low-cost, high-volume commercial product will be facilited through later participation of the staff at the Microelectronic Center of North Carolina (MCNC), Research Triangle, NC. A third task is to assemble a demultiplexer with control hardware/software and an advanced digital signal processing capability will be added to an existing workstation to support spike sorting from 256 channels or more. All three tasks will be done in parallel to incrementally add function to an existing, usable system. This complex, integrated project is made more feasib le by preliminary advances supported by NSF and elsewhere over past decade. This project aims to bring closur,9/to a decade-long development effort of the P.I. and his associates. The aim was to create and demonstrate the potential large scale neuron ensemble recording for decoding of information in patterns within distributed neuronal activity. Multivariate statistical methods have been applied to process the large volumes of data yielded by the new data acquisition methods. Recent publications by this group and others on multichannel recordings have created enthusiasm and momentum toward further advances in multichannel recording and analysis instrumentation. Advances in this capability are critical to enable a basic paradigm shift in the study of conceptual issues in the study of physiological neuronal networks.

系统神经生理学仪器开发的总体目标是创建功能设备，以便能够记录行为动物中非常大的神经元尖峰序列集合。该计划是开发和整合并行完成的三项任务。首先，将采用新的制造工艺来制造薄膜硅记录探针，每个探针有十六个位置。 探针形状和表面记录位点特性将针对长期慢性记录进行优化，以匹配 P.I. 之前完善的微线阵列的有效性。将在探针上图案化不同间距和尺寸的表面记录位点，并测试不同的记录表面。用于制造柔性微电缆和多芯片模块的新技术将适用于将信号从记录探针引导到外部信号处理电子器件。第二个任务是创建 VLSI 微芯片的设计，以检测和放大来自 16 个通道的信号。和多路信号到单个输出引线。该芯片还将提供具有控制功能的开关，以将测试/刺激应用于选定的输入。后续目标将是生产具有集成有源电子器件的多站点探头并创建多个探头组件。 待开发的工艺非常有利于商业生产方法。北卡罗来纳州三角研究区北卡罗来纳州微电子中心 (MCNC) 的工作人员稍后将参与其中，从而促进低成本、大批量的商业产品的开发。第三项任务是组装一个带有控制硬件/软件的解复用器，并将先进的数字信号处理功能添加到现有工作站中，以支持 256 个或更多通道的尖峰排序。所有三项任务将并行完成，以逐步向现有可用系统添加功能。 过去十年，美国国家科学基金会和其他机构支持的初步进展使这个复杂的综合项目变得更加可行。 该项目旨在结束 P.I. 长达十年的开发工作9/。 和他的同事。目的是创建并演示潜在的大规模神经元集成记录，用于解码分布式神经元活动中的模式信息。多元统计方法已应用于处理新数据采集方法产生的大量数据。该小组和其他人最近发表的有关多通道录音的出版物为多通道录音和分析仪器的进一步发展创造了热情和动力。这种能力的进步对于实现生理神经网络研究中概念问题研究的基本范式转变至关重要。