Large Scale Cortical Laminar Recordings: Novel Instrumentation

摘要 我们已经成功地设计并制造了用于清醒小鼠脊柱记录的背包驱动器 (AIM I)，并完全开发了一个新的行为模型AIM II(见Y1进度报告和Black ET Al.)。在这一补充应用中，我们提出了新的实验来阐明层流 SI中低频振荡的组织在比细胞定位更高的水平上 最初寻找的。这些新的实验将通过本地化来增强我们项目的整体影响 SI对特定板层的振荡及其与丘脑单单位放电的关系 生态相关和超快速伤害行为的背景。他们还将产生高 多个尺度(单个单位，LFP)的维度神经数据，可供其他单位使用 应调查人员的要求。

SUMMARY We have successfully designed and fabricated the backPack drive for spine recordings in awake mice (AIM I), and fully developed a novel behavioral model AIM II (see Y1 Progress Report and Black et al.). In this SUPPLEMENTAL APPLICATION, we propose new experiments to elucidate the laminar organization of low-frequency oscillations in SI at a much higher level of cellular localization than originally sought. These new experiments will augment the overall impact of our project by localizing oscillations in SI to specific lamina and correlating oscillations with thalamic single-unit firing in the context of ecologically-relevant and ultra-fast nociceptive behavior. They will also generate high dimensional neural data at multiple scales (single units, LFP) which will be available to other investigators upon request.