Large Scale Cortical Laminar Recordings: Novel Instrumentation

大规模皮质层流记录：新颖的仪器

• 批准号: 10078368
• 负责人: David Allenson Borton
• 金额: $ 8.09万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10078368
• 关键词: Award; Behavior; Behavioral; Behavioral Model; Behavioral Paradigm; Brain; Chronic; Custom; Data; Detection; Development; Electrophysiology (science); Fiber; Frequencies; Goals; Implant; Link; Lumbar spinal cord structure; Mediating; Mus; Nociception; Patient Self-Report; Preparation; Progress Reports; Reporting; Research Personnel; Rodent; Somatosensory Cortex; Spinal Cord; Stimulus; System; TRPV1 gene; Testing; Thalamic structure; Transgenic Mice; Vertebral column; awake; design; dorsal horn; experimental study; extracellular; high dimensionality; instrumentation; novel; optogenetics; relating to nervous system; tool development

SUMMARY We have successfully designed and fabricated the backPack drive for spine recordings in awake mice (AIM I), and fully developed a novel behavioral model AIM II (see Y1 Progress Report and Black et al.). In this SUPPLEMENTAL APPLICATION, we propose new experiments to elucidate the laminar organization of low-frequency oscillations in SI at a much higher level of cellular localization than originally sought. These new experiments will augment the overall impact of our project by localizing oscillations in SI to specific lamina and correlating oscillations with thalamic single-unit firing in the context of ecologically-relevant and ultra-fast nociceptive behavior. They will also generate high dimensional neural data at multiple scales (single units, LFP) which will be available to other investigators upon request.

概括 我们已经成功设计并制造了用于清醒小鼠脊柱录音的 backPack 驱动器 （AIM I），并全面开发了一种新颖的行为模型 AIM II（参见 Y1 进度报告和 Black et al.).在这个补充申请中，我们提出了新的实验来阐明层流 SI 中低频振荡的组织，其细胞定位水平比 originally sought.这些新实验将通过本地化来增强我们项目的整体影响 SI 中特定层板的振荡以及与丘脑单个单元放电相关的振荡 生态相关和超快速伤害感受行为的背景。他们还将产生高 多尺度（单个单元，LFP）的维度神经数据，可供其他人使用 调查员根据要求。