Large Scale Cortical Laminar Recordings: Novel Instrumentation

总结 我们已经成功地设计和制造了用于清醒小鼠脊柱记录的backPack驱动器 (AIM I），并完全开发了一种新的行为模型AIM II（见Y1进展报告和Black et 等）。在本实用新型中，我们提出了新的实验来阐明层流 在SI中低频振荡的组织在细胞定位的更高水平上， 最初寻求。这些新的实验将通过本地化， 振荡在SI到特定的层和相关的振荡与丘脑单单位放电在 生态相关和超快速伤害性行为的背景下。它们也将产生高 多维神经数据在多个尺度（单单位，LFP），这将提供给其他 调查人员要求。

SUMMARY We have successfully designed and fabricated the backPack drive for spine recordings in awake mice (AIM I), and fully developed a novel behavioral model AIM II (see Y1 Progress Report and Black et al.). In this SUPPLEMENTAL APPLICATION, we propose new experiments to elucidate the laminar organization of low-frequency oscillations in SI at a much higher level of cellular localization than originally sought. These new experiments will augment the overall impact of our project by localizing oscillations in SI to specific lamina and correlating oscillations with thalamic single-unit firing in the context of ecologically-relevant and ultra-fast nociceptive behavior. They will also generate high dimensional neural data at multiple scales (single units, LFP) which will be available to other investigators upon request.