Structural basis for ligand specificity and affinity in naturally occuring and artificial purine-binding RNA molecules

天然和人工嘌呤结合 RNA 分子中配体特异性和亲和力的结构基础

• 批准号: 107727641
• 负责人: Professor Dr. Jens Wöhnert
• 金额: --
• 依托单位: Institut für Molekulare Biowissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/107727641?language=en
• 关键词: Structural; basis; ligand; specificity; affinity

RNA molecules are able to bind to small molecule ligands with high affinity and specificity. The first examples for this were provide by artificially selected RNA-aptamers. Recently, it has been shown that direct RNA-small molecule interactions are also important in nature and regulate the expression of a significant number of bacterial genes through the action of riboswitches. The aim of this project is the structural comparison of novel riboswitches that bind to the purine nucleoside desoxyguanosine and/or the purine base guanine and of artificial RNA-aptamers that bind to similar ligands. We would like to characterize the possible diversity in RNA-ligand recognition modes and the relation between the structural complexity of RNA and ligand affinity and specificity. The results will have interesting implications for the design of drugs targeting RNA.

RNA分子能够以高亲和力和特异性结合小分子配体。第一个例子是由人工选择的 RNA 适体提供的。最近，研究表明，RNA-小分子的直接相互作用在自然界中也很重要，并通过核糖开关的作用调节大量细菌基因的表达。该项目的目的是对结合嘌呤核苷脱氧鸟苷和/或嘌呤碱基鸟嘌呤的新型核糖开关和结合相似配体的人工RNA适体进行结构比较。我们想要描述 RNA 配体识别模式可能的多样性以及 RNA 结构复杂性与配体亲和力和特异性之间的关系。这些结果将对 RNA 药物的设计产生有趣的影响。

项目成果

Mechanisms for differentiation between cognate and near-cognate ligands by purine riboswitches

嘌呤核糖开关区分同源配体和近同源配体的机制

• DOI: 10.4161/rna.20106
• 发表时间: 2012
• 期刊: RNA Biology
• 影响因子: 4.1
• 作者: A. Wacker;J. Buck;C. Richter;H. Schwalbe;J. Wöhnert
• 通讯作者: J. Wöhnert