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VPW: Cultivation of Cell-Polymer Tissue Constructs in Novel Bioreactors

VPW：在新型生物反应器中培养细胞聚合物组织结构

基本信息

批准号：
9627117
负责人：
Gilda Barabino
金额：
$ 11.76万
依托单位：
Massachusetts Institute of Technology
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1996
资助国家：
美国
起止时间：
1996-09-01 至 1998-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9627117&HistoricalAwards=false
关键词：
VPW Cultivation Cell Polymer Tissue

项目摘要

The overall objective of this research is to investigate the effect of the in vitro culture environment on the cultivation of cell- polymer constructs in bioreactors. Seeding mammalian cells onto biodegradable polymer scaffolds and growing tissues on these scaffolds in bioreactors represents a novel approach to tissue regeneration. Studies have shown that tissue growth and function is affected by environmental forces. In these studies, cell- polymer constructs are cultivated in three bioreactors with distinctly different fluid dynamic environments. Measurements are made using laser doppler and ultrasound doppler techniques to characterize the fluid dynamic environment in each reactor. These measurements are correlated with structural and functional tissue properties. Results from these studies will increase our basic understanding of tissue growth and function under various hydrodynamic conditions and contribute to the optimization of bioreactor systems for cultivation of clinically useful tissues. The interactive activities include developing and teaching an interactive tissue engineering course, coordinating a "Brown Bag Lunch" Series for women and minority faculty and students, coordinating an outreach program for K-12 students, and participating as a Faculty Role Model/Mentor in student activities administered by the New England Board of Higher Education.

本研究的总体目标是研究体外培养环境对生物反应器中细胞-聚合物构建体培养的影响。将哺乳动物细胞接种到生物可降解聚合物支架上并在生物反应器中在这些支架上生长组织代表了一种新的组织再生方法。研究表明，组织的生长和功能受到环境力量的影响。在这些研究中，细胞-聚合物构建体在具有明显不同的流体动力学环境的三个生物反应器中培养。使用激光多普勒和超声多普勒技术进行测量，以表征每个反应器中的流体动力学环境。这些测量与结构和功能组织特性相关。这些研究的结果将增加我们对各种流体动力学条件下组织生长和功能的基本理解，并有助于优化用于培养临床有用组织的生物反应器系统。互动活动包括开发和教学的互动组织工程课程，协调妇女和少数民族教师和学生的“布朗袋午餐”系列，协调K-12学生的推广计划，并作为教师榜样/导师参加由高等教育的新英格兰委员会管理的学生活动。