Matching Starter Grant awarded in conjunction with NSF Postdoctoral Fellowship in Plant Biology

与 NSF 植物生物学博士后奖学金联合颁发的配套启动补助金

• 批准号: 9628061
• 负责人: Daniel Vernon
• 金额: $ 0.35万
• 依托单位: Whitman College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-02-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9628061&HistoricalAwards=false
• 关键词: Matching; Starter; Grant; awarded; conjunction

The goal of this proposed research is to investigate genetic and molecular mechanisms underlying plant embryo development by characterizing embryo-defective (emb) mutants of Arabidopsis thaliana. Emphasis is on the characterization of the twn1 mutant, which is disrupted in embryonic morphogenesis and maintenance of cell type in the suspensor, and on emb88, a T-DNA insertion mutant that exhibits abnormal cell organization and arrested morphogenesis. Specific goals with twn1 include: 1) constructing double mutant lines with Arabidopsis mutants that have altered levels or sensitivity to plant growth regulators, and 2) characterizing the pleiotropic effects of the twn1 mutation on post embryonic development. These studies should provide insight into the function of the wild type TWN1 gene, and elucidate the mechanism of the dramatic embryogenic transformation observed in the mutant twn1 suspensor. Specific goals with emb88 include completing characterization of the EMB88 genomic locus and isolation of the EMB88 cDNA. This work should elucidate the biochemical function of the EMB88 gene product, and provide the foundation for a long term molecular study of the role of this gene in Arabidopsis embryogenesis. Molecular information on emb88 will have an impact on our understanding of Arabidopsis embryogenesis and emb mutants, because to date only a handful of Arabidopsis embryo mutants have been characterized at the molecular level. Together, twn1 and emb88 should yield valuable information on key processes of plant embryogenesis, including morphogenesis, maintenance of differentiated cell type, and intercellular communication in the developing embryo.

本研究的目的是通过描述拟南芥胚胎缺陷突变体来研究植物胚胎发育的遗传和分子机制。重点是对twn 1突变体的表征，这是破坏胚胎形态发生和维持细胞类型的胚柄，和emb 88，T-DNA插入突变体，表现出异常的细胞组织和逮捕形态发生。twn 1的具体目标包括：1）构建具有对植物生长调节剂具有改变的水平或敏感性的拟南芥突变体的双突变体系，以及2）表征twn 1突变对胚胎后发育的多效性效应。这些研究应提供深入了解野生型TWN 1基因的功能，并阐明突变体TWN 1胚柄中观察到的戏剧性的胚胎发生转化的机制。emb 88的具体目标包括完成EMB 88基因组位点的表征和EMB 88 cDNA的分离。本研究为阐明EMB 88基因产物的生物化学功能奠定了基础，并为进一步研究EMB 88基因在拟南芥胚胎发生中的作用奠定了基础。emb 88的分子信息将对我们理解拟南芥胚胎发生和emb突变体产生影响，因为迄今为止，只有少数拟南芥胚胎突变体已在分子水平上的特点。twn 1和emb 88结合在一起，将为植物胚胎发生的关键过程提供有价值的信息，包括形态发生、分化细胞类型的维持和发育中胚胎的细胞间通讯。