Resolving Arthropod Phylogeny Using Multiple Nuclear Genes

使用多个核基因解析节肢动物系统发育

• 批准号: 9629791
• 负责人: Jeffrey Shultz
• 金额: $ 12.5万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-15 至 2000-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9629791&HistoricalAwards=false
• 关键词: Resolving; Arthropod; Phylogeny; Using; Multiple

9629791 Shultz Because they encompass an unparalleled range of structural and taxonomic diversity and have an extremely rich and ancient fossil record, the arthropods (spiders, crabs, insects and their relatives) offer many opportunities for addressing fundamental issues in evolutionary biology. Any meaningful synthesis requires a firm understanding of how the arthropods are related to one another; but the phylogenetic relationships among the major arthropod lineages are difficult to recover. Many different and controversial reconstructions have been proposed. In this study, Jeffrey W. Schultz and Jerome C. Regier will determine whether analysis of the DNA sequences of two slowly-evolving nuclear genes, elongation factor 1a and RNA polymerase II, can provide a more robust phylogenetic reconstruction for arthropods. The researchers will obtain and sequence these genes from a large sample of arthropods representing all major groups, and will analyze these sequences using phylogenetic reconstruction methods. Studies on other organisms have suggested that these genes are appropriate for the analysis of lineages as anciently diverged as the arthropod lineages are thought to be. A more fully resolved phylogenetic tree for arthropods will offer a unique and useful framework for addressing basic questions about the tempo and mode of phylogenetic and structural diversification in arthropods and in other forms of life.

9629791 Shultz 由于节肢动物（蜘蛛、螃蟹、昆虫及其近亲）具有无与伦比的结构和分类多样性，并且拥有极其丰富和古老的化石记录，因此为解决进化生物学中的基本问题提供了许多机会。 任何有意义的综合都需要对节肢动物之间的关系有深入的了解；但主要节肢动物谱系之间的系统发育关系很难恢复。 人们提出了许多不同且有争议的重建方案。 在这项研究中，Jeffrey W. Schultz 和 Jerome C. Regier 将确定对两个缓慢进化的核基因（延伸因子 1a 和 RNA 聚合酶 II）的 DNA 序列进行分析是否可以为节肢动物提供更稳健的系统发育重建。 研究人员将从代表所有主要类群的大量节肢动物样本中获取这些基因并对其进行测序，并将使用系统发育重建方法分析这些序列。 对其他生物体的研究表明，这些基因适用于分析像节肢动物谱系一样古老的谱系。 更全面解析的节肢动物系统发育树将为解决有关节肢动物和其他生命形式的系统发育和结构多样化的节奏和模式的基本问题提供一个独特且有用的框架。