Role of Pto- and Fen-interacting Proteins in Plant Disease Resistance and Fenthion Sensitivity

Pto 和 Fen 相互作用蛋白在植物抗病性和倍硫磷敏感性中的作用

• 批准号: 9630635
• 负责人: Gregory Martin
• 金额: $ 23.1万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1998-08-13
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630635&HistoricalAwards=false
• 关键词: Role; Pto; Fen; interacting; Proteins

Abstract 9630635 Martin The goal of this research is to gain a detailed understanding of pathogen recognition and signal transduction events involved in plant disease resistance. Two experimental systems in tomato are being studied, resistance to Pseudomonas syringae pv. tomato (causative agent of bacterial speck disease) conferred by the Pto gene and sensitivity to the fenthion insecticide conferred by the Fen gene. The Pto and Fen genes are members of a small gene family encoding closely related serine/threonine protein kinases. In the proposed research several genes that have been shown to encode proteins that physically interact with the Pto kinase will be characterized. These proteins will be examined using biochemical techniques and transgenic approaches. In addition, genes will be identified which are regulated by the Pto and Fen kinases, and they will used to examine the pathways whereby plants defend themselves from pathogens. These studies will aid in our understanding of the molecular basis of recognition and signal transduction events involved in plant defense against pathogens. ***

摘要：本研究的目的是详细了解植物抗病过程中涉及的病原体识别和信号转导事件。目前正在研究两种番茄抗丁香假单胞菌试验体系。由Pto基因决定的番茄（细菌性斑点病的病原体）和由Fen基因决定的对倍硫磷杀虫剂的敏感性。Pto和Fen基因是一个小基因家族的成员，编码密切相关的丝氨酸/苏氨酸蛋白激酶。在提出的研究中，几个基因已被证明编码蛋白质，与Pto激酶的物理相互作用将被表征。这些蛋白质将使用生化技术和转基因方法进行检测。此外，还将鉴定受Pto和Fen激酶调控的基因，并将它们用于检查植物保护自己免受病原体侵害的途径。这些研究将有助于我们了解植物防御病原体的识别和信号转导事件的分子基础。＊＊＊