Role of MAPKKKa-Mediated Cell Death in Plant Disease Resistance and Susceptibility

MAPKKKa 介导的细胞死亡在植物抗病性和易感性中的作用

• 批准号: 0444600
• 负责人: Gregory Martin
• 金额: $ 44万
• 依托单位: Boyce Thompson Institute Plant Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0444600&HistoricalAwards=false
• 关键词: Role; MAPKKKa; Mediated; Cell; Death

Role of MAPKKKa-mediated cell death in plant disease resistance and susceptibilityGregory Martin, Boyce Thompson Institute for Plant ResearchLocalized cell death occurs in leaves of both disease-resistant and disease-susceptible plants upon attack by disease-causing organisms. In resistant plants, rapid cell death (6 hrs) is referred to as the 'hypersensitive response' and is thought to inhibit further spread of the pathogen. In susceptible plants, host cell death occurs over the course of many days and manifests as disease symptoms referred to as 'specks', 'spots', or 'blights'. The extent to which host cell death is either a consequence or a cause of disease resistance and susceptibility is a central unresolved question in the study of plant pathogen interactions - in part because the molecular mechanisms underlying pathogenesis-related cell death are largely unknown. To investigate host cell death, this project will investigate both resistance and susceptibility in tomato to bacterial speck disease caused by Pseudomonas syringae pv. tomato. The central focus of the work will be a gene that encodes MAP kinase kinase kinase (MAPKKKa). When expression of this gene is interfered with the plant does not develop cell death during a disease-resistance response. Unexpectedly, it was found that silencing of MAPKKKa also interferes with cell death associated with bacterial speck disease. These observations indicate that the host plays an active role in promoting the formation of disease symptoms and that MAPKKKa acts as a molecular switch governing cell death associated with both resistance and susceptibility.This project aims to further define the molecular mechanisms underlying the role of MAPKKKa in host cell death. The objectives are: 1) Identify and characterize host proteins that function upstream of MAPKKKa; 2) Investigate the extent to which host disease resistance and susceptibility pathways share the same signaling components; and 3) Examine the role of MAPKKKa-mediated cell death in host responses to biotrophic and necrotrophic pathogens.There are three areas where this research will have broader impact: 1) Undergraduate students and high school students will be trained as part of the NSF-supported Plant Genomics Summer Research Program hosted by the Boyce Thompson Institute; 2) The PI and members of his lab will continue their involvement in several outreach programs including the New Visions high school program; and 3) Deeper understanding of the role of host cell death during pathogen attack will facilitate the development of new strategies of disease control thereby lessening dependence on pesticides.

MAPKKKa 介导的细胞死亡在植物抗病性和易感性中的作用格雷戈里·马丁，博伊斯·汤普森植物研究所在受到致病生物体攻击时，局部细胞死亡发生在抗病和易感植物的叶子中。 在抗性植物中，快速细胞死亡（6小时）被称为“过敏反应”，并被认为可以抑制病原体的进一步传播。 在易感植物中，宿主细胞死亡会持续许多天，并表现为称为“斑点”、“斑点”或“枯萎病”的疾病症状。 宿主细胞死亡在多大程度上是抗病性和易感性的结果或原因，是植物病原体相互作用研究中一个未解决的核心问题——部分原因是与发病机制相关的细胞死亡的分子机制在很大程度上是未知的。 为了研究宿主细胞死亡，该项目将研究番茄对由丁香假单胞菌引起的细菌性斑点病的抗性和易感性。番茄。 这项工作的中心焦点是编码 MAP 激酶激酶 (MAPKKKa) 的基因。 当该基因的表达受到干扰时，植物在抗病反应期间不会发生细胞死亡。 出乎意料的是，我们发现 MAPKKKa 的沉默也会干扰与细菌性斑点病相关的细胞死亡。 这些观察结果表明，宿主在促进疾病症状的形成中发挥着积极作用，并且 MAPKKKa 作为控制与抵抗性和易感性相关的细胞死亡的分子开关。该项目旨在进一步明确 MAPKKKa 在宿主细胞死亡中作用的分子机制。 目标是： 1) 鉴定并表征在 MAPKKKa 上游发挥作用的宿主蛋白； 2) 研究宿主抗病和易感途径共享相同信号成分的程度； 3) 研究 MAPKKKa 介导的细胞死亡在宿主对生物营养型和坏死性营养型病原体的反应中的作用。这项研究将在三个领域产生更广泛的影响：1) 本科生和高中生将接受培训，作为博伊斯·汤普森研究所主办的 NSF 支持的植物基因组学夏季研究计划的一部分； 2) PI及其实验室成员将继续参与多个外展项目，包括新视野高中项目； 3）更深入地了解宿主细胞死亡在病原体攻击过程中的作用将有助于制定新的疾病控制策略，从而减少对农药的依赖。