Molecular Genetics of Disease Resistance in Tomato

番茄对假单胞菌番茄致病变种（Pst）的抗性是 由一个单一的显性基因座Pto赋予。所需工具 克隆该基因座是在植物的使用期内开发的 博士后奖学金。 番茄近等基因系是 用于鉴定与该基因紧密连锁的RFLP和RAPD标记 大量的F2群体被用来创建高分辨率的 连锁图，允许精确定位Pto相对于 分子标记构建了番茄YAC文库， 筛选确定了Pto基因座的候选者。该项目将 继续和推定的Pto基因分离和表征。 %%% 这项工作应该大大增加我们的理解， 控制植物病害的能力。

Resistance to Pseudomonas syringae pv.tomato(Pst) in tomato is conferred by a single dominant locus, Pto. Tools necessary for cloning the locus were developed during the tenure of the Plant Postdoctoral Fellowship Award. Near Isogenic lines of tomato were used to identify RFLP and RAPD markers tightly linked to the gene and a large F2 population was used to create a high resolution linkage map that allowed precise placement of Pto in relation to the molecular markers. A tomato YAC library was constructed and a screen identified a candidate for the Pto locus. The project will be continued and the putative Pto gene isolated and characterized. %%% This work should significantly increase our understanding and ability to control disease in plants.