Analysis of a Tomato Pseudomonas Resistance Locus, Pto

番茄假单胞菌抗性位点 Pto 的分析

• 批准号: 9303359
• 负责人: Gregory Martin
• 金额: $ 32万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-15 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9303359&HistoricalAwards=false
• 关键词: Analysis; Tomato; Pseudomonas; Resistance; Locus

9303359 Martin The long term objective of this research is to elucidate the function of plant disease resistance (DR) genes. The interaction of tomato and the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) provides an ideal system for understanding gene-for- gene complementarity. Resistance to Pst in tomato is conferred by a single, mapped locus, Pto and an avr gene corresponding to Pto has been isolated (avrPto). A multi-step positional cloning strategy has recently been used to identify a yeast artificial chromosome (YAC) clone that contains the Pto locus. We now propose to molecularly isolate the Pto locus from this YAC clone and characterize its role in resistance. We will isolate the Pto bacterial resistance locus; determine the DNA sequence of Pto and examine its structural features; investigate potential alternate alleles of Pto that exist in susceptible plants; characterize the spatial and temporal expression patterns of the Pto gene; examine the expression patterns of pathogenesis-related (PR) genes and other defense-related using Pto isogenic lines, and determine the spatial and temporal accumulation patterns of the Pto protein. %%% Knowledge of the DNA sequence of the Pto gene and the localization of its gene product in the plant cell will address fundamental questions in cell-cell interactions. In addition, since the Pto gene will likely be one of the first plant resistance genes isolated, its characterization may facilitate the isolation of disease resistance genes from other plant species. ***

9303359 Martin 这项研究的长期目标是阐明植物抗病（DR）基因的功能。 番茄与细菌病原体丁香假单胞菌的相互作用。 番茄（Pst）为理解基因间互补性提供了一个理想的系统。 番茄对 Pst 的抗性是由单个定位基因座 Pto 赋予的，并且已分离出与 Pto 相对应的 avr 基因 (avrPto)。 最近使用多步定位克隆策略来鉴定包含 Pto 基因座的酵母人工染色体 (YAC) 克隆。 我们现在建议从该 YAC 克隆中分子分离 Pto 基因座，并表征其在抗性中的作用。 我们将分离出Pto细菌耐药位点；确定Pto的DNA序列并检查其结构特征；研究易感植物中存在的 Pto 潜在替代等位基因；表征 Pto 基因的空间和时间表达模式；使用 Pto 同基因系检查发病相关 (PR) 基因和其他防御相关基因的表达模式，并确定 Pto 蛋白的空间和时间积累模式。 %%% 了解 Pto 基因的 DNA 序列及其基因产物在植物细胞中的定位将解决细胞间相互作用的基本问题。 此外，由于Pto基因可能是最早分离的植物抗性基因之一，因此其表征可能有助于从其他植物物种中分离抗病基因。 ***