Collaborative Research: Recombinant Production of Enzymes from Hyperthermophiles: Effects of High Temperature and Pressure

合作研究：从超嗜热菌中重组生产酶：高温高压的影响

• 批准号: 9632554
• 负责人: Frank Robb
• 金额: --
• 依托单位: University of Maryland Biotechnology Institute
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-15 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9632554&HistoricalAwards=false
• 关键词: Collaborative; Research; Recombinant; Production; Enzymes

9632554 Robb This proposal is on research to examine two key issues in the development of enzymes and other proteins from thermophilic microorganisms which are to: (1) develop processing methods to obtain significant quantities of these enzymes for further investigation and potential application, and (2) examine the mechanisms of pressure-induced stabilization of proteins from extreme thermophiles and devise approaches to manipulate the thermostability of these enzymes. Site-directed mutagenesis is to be used in a systematic way, along with biocatalytic studies at elevated pressures to elucidate the effects of pressure on protein thermostability and the interaction of subunits in these multimeric enzymes. ***

9632554 Robb该提案旨在研究从嗜热微生物中开发酶和其他蛋白质的两个关键问题，即：（1）开发加工方法以获得大量的这些酶用于进一步研究和潜在应用，和（2）研究压力诱导的极端嗜热菌蛋白质稳定化的机制，并设计方法来操纵这些酶的热稳定性。 定点诱变将以系统的方式使用，沿着在升高的压力下的生物催化研究，以阐明压力对蛋白质热稳定性的影响和这些多聚体酶中亚基的相互作用。 ***