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Ammonium sensing by plant roots

植物根部感测铵

基本信息

批准号：
72169357
负责人：
Professor Dr. Nicolaus von Wirén
金额：
--
依托单位：
Abteilung Physiologie und Zellbiologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2008
资助国家：
德国
起止时间：
2007-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/72169357?language=en
关键词：
Ammonium sensing plant roots

项目摘要

The sensing of localized nutrient patches in soils by plant roots is not only part of a strategy to increase the competitiveness of plants in natural habitats but also important in agricultural production systems to improve fertilizer use efficiency of crops. Due to the high responsiveness of lateral roots to the localized supply of certain nutrients, lateral root formation has been established as a morphological read-out for nutrient sensing. During the first funding period, we have shown that localized ammonium supply triggers higher-order lateral root branching whereas nitrate stimulates lateral root elongation. In Arabidopsis, this morphological response is mediated by the ammonium transporter AMT1;3. The aim of the present proposal is i) to investigate whether AMT1;3 acts as an ammonium transceptor coupling the ammonium transport process with a signaling event leading to lateral root branching; ii) to determine the role of the phytohormone auxin in ammonium-induced lateral root branching, and iii) to identify genes in the ammonium signaling pathway downstream of the ammonium transport process from a set of genes identified in transcriptome approaches. Our approach includes AMT promoter swapping experiments for expression in a quadrouple amt knock-out line (qko) and root system analysis on split-agar plates with localized ammonium supplies. Moreover, qko lines with reconstituted expression of individual AMTs will be transformed with auxin reporter constructs and analyzed for expression of candidate genes. The ultimate goal is to identify molecular components in Arabidopsis roots integrating the information on a locally restricted ammonium source into the root developmental program.

通过植物根部感知土壤中的局部养分斑块不仅是提高自然栖息地植物竞争力的策略的一部分，而且在农业生产系统中对于提高作物肥料利用效率也很重要。由于侧根对某些养分的局部供应具有高度响应性，侧根形成已被确立为养分传感的形态读数。在第一个资助期间，我们已经表明，局部铵供应触发高阶侧根分枝，而硝酸盐则刺激侧根伸长。在拟南芥中，这种形态反应是由铵转运蛋白 AMT1 介导的；3。本提案的目的是 i) 研究 AMT1;3 是否充当铵受体，将铵转运过程与导致侧根分枝的信号事件耦合起来； ii) 确定植物激素生长素在铵诱导的侧根分枝中的作用，以及 iii) 从转录组方法鉴定的一组基因中鉴定铵转运过程下游的铵信号通路中的基因。我们的方法包括 AMT 启动子交换实验，以在四重 amt 敲除系 (qko) 中表达，以及在具有局部铵供应的分裂琼脂板上进行根系统分析。此外，具有单个 AMT 重建表达的 qko 系将用生长素报告构建体进行转化，并分析候选基因的表达。最终目标是识别拟南芥根中的分子成分，将局部限制的铵源信息整合到根发育程序中。