Collaborative Research: Metabolic Manipulation of Taxas sp. Cell Culture for Taxane Production

合作研究：类群的代谢操纵。

• 批准号: 9702582
• 负责人: James Linden
• 金额: $ 12.11万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-15 至 1999-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9702582&HistoricalAwards=false
• 关键词: Collaborative; Research; Metabolic; Manipulation; Taxas

9702582 Linden The Principal Investigators (PIs) propose to fill the need for a more rational approach to optimization of production conditions for new compounds derived from plant cell cultures. They plan to exploit evolving knowledge of signal- transduction pathways in plants with experiments using metabolic transport inhibitors, precursors, and signaling compounds (e.g. elicitors and plant hormones). This approach is to lead to working models of TAXOL synthesis, release, and degradation in Taxus sp. cell cultures. The PIs believe the proposed approach should be generalizable to compounds other than TAXOL that are derived from plant cell cultures, and provide biochemical engineers a tool to achieve rapid production of novel therapeutic compounds from plant cells. ***

9702582林登主要研究者（PI）提出了一种更合理的方法来优化植物细胞培养物衍生的新化合物的生产条件，以满足这一需求。 他们计划利用植物中信号转导途径的不断发展的知识，使用代谢转运抑制剂，前体和信号化合物（例如激发子和植物激素）进行实验。 这种方法将导致紫杉醇的合成，释放和降解的红豆杉细胞培养的工作模型。 PI认为，拟议的方法应该可推广到除TAXOL之外的来自植物细胞培养物的化合物，并为生化工程师提供一种工具，以实现从植物细胞快速生产新型治疗化合物。 ***