High-Resolution Low Temperature SEM

要求提供经费以购置一台高分辨率低温扫描电子显微镜。 仪器为日立S5000型扫描电子显微镜，配有冷冻系统、背散射电子探测器、数字图像系统，并可远程操作。 使用低温显微镜，样品在检查过程中保持在液氮温度下，允许水被保存以用于冷冻水合状态的成像，并且细胞的表面和内部被分析而不需要化学固定，脱水或干燥。

Funds are requested for a high-resolution low-temperature scanning electron microscope. The instrument isan Hitachi S5000 scanning electron microscope with cryosystem, backscattered electron detector, digital image system, and is matrixed for remote operation. With low-temperature microscopy the sample is held at liquid nitrogen temperature during examination, allowing water to be preserved for imaging in the frozen hydrated state and the surface and interior of cells to be analyzed without the need for chemical fixation, dehydration or drying.