CAA: Developmental Genetic Analysis of an Eyeless Strain of Mice. A Study Toward Understanding Eye Morphogenesis and Evolution

CAA：无眼小鼠品系的发育遗传分析。

• 批准号: 9707451
• 负责人: Priscilla Tucker
• 金额: $ 4.2万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9707451&HistoricalAwards=false
• 关键词: CAA; Developmental; Genetic; Analysis; Eyeless

Abstract Priscilla Tucker CAA 9707451 The proposed research is designed to identify genes responsible for the early stages of eye development in mammals. The project has two aims. First, a genetic analysis of an eyeless mutant mouse strain will be carried out to identify one or more genes responsible for the eyeless condition. This involves conducting a genetic mapping study in which eyeless mice are crossed with normal mice. Differences in DNA content between normal and eyeless descendants of the cross are localized to specific chromosomes and candidate genes, those thought to be responsible for the eyeless condition, are identified. Second, a comparative study of gene expression will be conducted in both eyeless and normal mice. Patterns of gene expression for nine genes known to be involved in the early development of the eye will be compared and any differences in expression of these genes between eyeless and normal mice will be noted. The project has important implications for furthering an understanding of how the mammalian eye develops as it will provide new information on what genes are responsible for eye development and when and where they are expressed during eye development. In addition, identification of one or more genes responsible for the eyeless condition in these mice may shed light on the genetic basis of instances of eyelessness in nature. More generally, the project has important implications for understanding how eyes evolved in animals. In nature, different species of animals exhibit a great diversity of eye types and, for years, researchers thought that eyes evolved independently multiple times. Yet, recently, several genes involved in eye development are found in such diverse organisms as fruit flys and humans. This observation suggests that animal eyes have a common developmental origin. The search for new genes involved in eye development in animals will further our understanding of how the same genes can be utilized differently in different organisms to produce the great diversity of eye types found in nature.

摘要 Priscilla Tucker 9707451 这项研究旨在确定哺乳动物眼睛发育早期阶段的基因。 该项目有两 目标。 首先，将对一种无眼突变小鼠品系进行遗传分析。 以确定一个或多个基因负责无眼 条件 这涉及到进行基因图谱研究， 将无眼小鼠与正常小鼠杂交。 DNA含量的差异 正常和无眼的杂交后代之间的差异， 特定的染色体和候选基因，那些被认为是负责 对于失明的情况，被识别。 第二，比较研究 将在无眼小鼠和正常小鼠中进行基因表达的研究。 已知参与肿瘤生长的9个基因的基因表达模式 将比较眼睛的早期发育， 将注意到这些基因在无眼小鼠和正常小鼠之间的表达。 该项目对进一步了解哺乳动物眼睛的发育具有重要意义，因为它将为人类提供新的 关于哪些基因负责眼睛发育以及何时和 在眼睛发育过程中表达。 此外，本发明还提供了一种方法， 鉴定一个或多个导致失明的基因 在这些小鼠中，可能揭示了遗传基础的实例， 自然界中的无眼 更一般地说，该项目具有重要的 对理解动物眼睛进化的意义。 在自然界中， 不同种类的动物表现出眼睛类型的巨大差异， 多年来，研究人员认为眼睛是独立进化的， 次 然而，最近发现了几个与眼睛发育有关的基因， 在果蝇和人类这样的多种生物中。 该观察结果 表明动物的眼睛有着共同的发育起源。 搜索 在动物眼睛发育过程中发现新的基因， 了解相同的基因如何被不同地利用， 不同的生物产生了巨大的眼睛类型的多样性， 自然