Developmental and genetic dynamics of ambrosia beetle-fungal partner symbioses
豚草甲虫-真菌伴侣共生的发育和遗传动力学
基本信息
- 批准号:2241029
- 负责人:
- 金额:$ 76.54万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-04-01 至 2024-02-29
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Ambrosia beetles infest trees, where they dig into the bark to lay their eggs. These beetles are unique in that they also harbor fungi that act as their food source which grows on the tree tissues. Some of these fungi can then infect the tree and in several cases can result in the death of host trees within months of infection. These beetles and their pathogenic fungi occur worldwide and pose a significant threat to forestry and natural ecosystems. However, no genetic models for the study of any ambrosia beetle-fungal partner pairings are available. This proposal will use the Xyloborus beetle-laurel wilt pathogenic fungus (Harringtonia lauricola) as a model. Both the original beetle and the fungus are invasive to the United States and are rapidly spreading throughout the Southeastern part of the country. The beetle carries the fungus to trees, where the fungus is capable of killing otherwise healthy trees. There are over 500 different species of trees that the beetle uses as a host including swampbay, redbay, sassafras, and the agriculturally important crop of avocado, where the only treatment is to cut and burn the trees. In this project, we will study the dynamics and genetics of how the beetle harbors its fungal partner, including how the beetle chooses, promotes the growth of, and carries the fungus from host tree to host tree. These data can provide foundational information that can ultimately be used to help stop the spread of these tree pathogens. In addition, the project will provide opportunities for training and outreach to high school, undergraduate, graduate, and postdoctoral students on fungal biology, disease, symbioses and evolutionary processes. In partnership with programs at University of Florida, the investigator will develop educational material for the high school teachers and the general public on fungal-insect symbioses. Insect-fungal mutualisms are widespread in nature and ambrosia beetle-fungal interactions represent some of the oldest and most successful symbioses known. The fungus serves as the sole source of nutrition for the beetles that burrow into trees to form galleries where they grow and “farm” the fungus for food. These beetles have evolved specialized structures (mycangia) that house, transport and disseminate the fungus. These fungi and beetles have co-adapted to symbiotic lifestyles throughout evolutionary history and include independent evolutions of different types of mycangia that house different fungal symbionts. The fungus, Harringtonia lauricola is a highly destructive tree pathogen affecting avocado and other members of the Lauraceae family and has killed 300 million trees in the US alone. H. lauricola is the mutualistic partner to its original invasive (to the US) beetle carrier (Xyleborus glabratus) and may have now spread to indigenous beetle species. We will investigate the developmental and genetic mechanisms which underlie the relationship between H. lauricola and Xyleborus beetles. The project will use cellular and genetic approaches to test specific hypotheses concerning symbiotic association. We will: (1) probe the cellular dynamics of mycangial colonization, including investigating selection, persistence/maintenance, turnover, and competition in the mycangia using microbiological and cellular (e.g., microscopic) methods, (2) identify genetic mechanisms that mediate fungal symbioses, e.g., what fungal genes/network are important for colonization and fitness within the mycangia, and (3) construct gene expression profiles (i.e., transcriptomics) of the fungus during mycangial colonization to help define the evolutionary processes that has led to fungal-mycangial symbioses.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
豚草甲虫在树上出没,它们钻到树皮里产卵。这些甲虫的独特之处在于它们还携带真菌,作为它们在树木组织上生长的食物来源。这些真菌中的一些可以感染树木,在某些情况下可以导致宿主树木在感染后几个月内死亡。这些甲虫及其致病真菌在世界范围内发生,对林业和自然生态系统构成重大威胁。然而,没有遗传模型的研究任何豚草甲虫真菌的合作伙伴配对。该建议将使用Xyloborus beetle-laurel枯萎病病原真菌(Harringtonia lauricola)作为模型。最初的甲虫和真菌都是美国的入侵者,并迅速蔓延到美国东南部。甲虫将真菌带到树上,在那里真菌能够杀死健康的树木。有超过500种不同的树木被甲虫用作宿主,包括沼泽贝、红贝、黄樟和农业上重要的鳄梨作物,唯一的治疗方法是砍伐和烧毁树木。在这个项目中,我们将研究甲虫如何窝藏其真菌伴侣的动力学和遗传学,包括甲虫如何选择,促进生长,并将真菌从宿主树带到宿主树。这些数据可以提供基本信息,最终可以用来帮助阻止这些树木病原体的传播。此外,该项目将为高中生、本科生、研究生和博士后提供真菌生物学、疾病、共生和进化过程方面的培训和推广机会。研究人员将与佛罗里达大学的项目合作,为高中教师和公众开发关于真菌-昆虫共生的教育材料。 昆虫-真菌互惠共生在自然界中广泛存在,豚草甲虫-真菌的相互作用代表了一些已知的最古老和最成功的共生关系。这种真菌是甲虫唯一的营养来源,甲虫钻到树上形成画廊,在那里它们生长并“种植”真菌作为食物。这些甲虫已经进化出专门的结构(mycangia),可以容纳,运输和传播真菌。这些真菌和甲虫在整个进化历史中共同适应了共生的生活方式,并且包括容纳不同真菌共生体的不同类型的真菌囊的独立进化。真菌Harringtonia lauricola是一种高度破坏性的树木病原体,影响鳄梨和樟科家族的其他成员,仅在美国就杀死了3亿棵树。H. lauricola是其最初的入侵(美国)甲虫载体(Xyleborus glabratus)的互惠伙伴,现在可能已经传播到本地甲虫物种。我们将探讨H. lauricola和Xyleborus甲虫。该项目将使用细胞和遗传方法来测试有关共生关系的特定假设。我们将:(1)探测真菌囊定殖的细胞动力学,包括使用微生物和细胞(例如,显微)方法,(2)鉴定介导真菌共生的遗传机制,例如,什么真菌基因/网络对于真菌囊内的定殖和适应性是重要的,和(3)构建基因表达谱(即,该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
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Nemat Keyhani其他文献
Have biopesticides come of age?
- DOI:
10.1016/j.tibtech.2012.01.003 - 发表时间:
2012-05-01 - 期刊:
- 影响因子:
- 作者:
Travis Glare;John Caradus;Wendy Gelernter;Trevor Jackson;Nemat Keyhani;Jürgen Köhl;Pamela Marrone;Louise Morin;Alison Stewart - 通讯作者:
Alison Stewart
Nemat Keyhani的其他文献
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{{ truncateString('Nemat Keyhani', 18)}}的其他基金
Developmental and genetic dynamics of ambrosia beetle-fungal partner symbioses
豚草甲虫-真菌伴侣共生的发育和遗传动力学
- 批准号:
2418026 - 财政年份:2023
- 资助金额:
$ 76.54万 - 项目类别:
Standard Grant
Mechanisms of Mutualism and Parasitism: Entomopathogenic Fungi, Insects, and Plants
共生和寄生机制:昆虫病原真菌、昆虫和植物
- 批准号:
1557704 - 财政年份:2016
- 资助金额:
$ 76.54万 - 项目类别:
Continuing Grant
Breaching the insect waxy layer: host pathogen interactions and epicuticle degradation by entomopathogenic fungi
突破昆虫蜡质层:宿主病原体相互作用和昆虫病原真菌的表皮降解
- 批准号:
1121392 - 财政年份:2011
- 资助金额:
$ 76.54万 - 项目类别:
Continuing Grant
REU Site: Fueling a Microbiology Research Training Network from the University of Florida
REU 网站:推动佛罗里达大学微生物学研究培训网络
- 批准号:
0649198 - 财政年份:2007
- 资助金额:
$ 76.54万 - 项目类别:
Continuing Grant
Microbial Genome Sequencing: Comparative Analysis of In vivo and In vitro Transcripts (ESTs) of Beauveria bassiana
微生物基因组测序:白僵菌体内和体外转录本 (EST) 的比较分析
- 批准号:
0412137 - 财政年份:2004
- 资助金额:
$ 76.54万 - 项目类别:
Standard Grant
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