RUI: RNA Stability and Ion Binding

RUI：RNA 稳定性和离子结合

• 批准号: 9707940
• 负责人: Martin Serra
• 金额: $ 10.42万
• 依托单位: Allegheny College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9707940&HistoricalAwards=false
• 关键词: RUI; RNA; Stability; Ion; Binding

Serra 9707940 The goal of this project is to determine the effect of sequence and divalent ions on the stability of RNA. This project has four specific goals: 1) to test the limits of a model for the prediction of RNA hairpin stability, 2) to provide the thermodynamics parameters for the prediction of RNA bulge loop stability from sequence, 3) to determine the effect of metal ions on the stability of RNA secondary structural motifs, and 4) to determine the stoichiometry and affinity for metal ion binding to RNA secondary structural motifs. RNA oligomers will be prepared by chemical synthesis. The structural stability of the RNA oligomers will be determined by Van't Hoff analysis of optical melting curves of the synthetic oligomers. Interaction of metal ions (Mg2+ and Zn2+) with RNA will be determined by observing their effect on the thermal stability of RNA oligomers in optical melting studies. The stoichiometry and affinity of the metal ion binding will be determined by equilibrium dialysis. The overall goal of this research is to determine the effect of sequence on the structural stability of RNA oligomers and to understand the molecular forces responsible for their stability and to provide thermodynamic parameters for the prediction of those stabilities. ***

这个项目的目标是确定序列和二价离子对RNA稳定性的影响。本项目有四个具体目标：1)测试预测RNA发夹稳定性模型的极限；2)从序列上为预测RNA凸环稳定性提供热力学参数；3)确定金属离子对RNA二级结构基序稳定性的影响；4)确定金属离子与RNA二级结构基序结合的化学计量学和亲和力。RNA低聚物将通过化学合成制备。RNA低聚物的结构稳定性将通过合成低聚物的光学熔化曲线的范霍夫分析来确定。金属离子（Mg2+和Zn2+）与RNA的相互作用将在光学熔融研究中通过观察它们对RNA低聚物热稳定性的影响来确定。金属离子结合的化学计量学和亲和力将通过平衡透析来确定。本研究的总体目标是确定序列对RNA低聚物结构稳定性的影响，了解影响其稳定性的分子力，并为预测这些稳定性提供热力学参数。＊＊＊