RUI: RNA Stability and Metal Ion Bonding

这个研究项目的最终目标是表征负责RNA结构稳定性的分子相互作用。二价金属离子（Mg +2）在核酸生物化学中所起的重要作用表明，需要更好地了解二价金属离子对RNA结构稳定性的依赖。该项目的具体目的是研究金属离子（特别是Mg +2）与小而结构明确的RNA低聚物的结合。将研究金属离子与常见RNA二级结构基序（即双工、发夹、末端错配、凸起和内环）的相互作用。本研究旨在确定生理条件下金属离子与这些RNA结构基序结合的理化参数。这些研究将结合热稳定性和结合研究，以更好地了解rna -金属相互作用的本质。

MCB 0075962Serra, Martin The ultimate goal of this research project is to characterize the molecular interactions responsible for the structural stability of RNA. The fundamental role played by divalent metal ions (Mg +2 ) in nucleic acid biochemistry highlights the need to understand better the dependence of divalent metal ions on RNA structural stability. The specific aim of this project is to investigate metal ion binding (specifically Mg +2 ) to small and structurally well-defined RNA oligomers. The interaction of metal ions with common RNA secondary structural motifs (i.e. duplex, hairpin, terminal mismatch, bulge and internal loop) will be investigated. This study is designed to determine the physiochemical parameters underlying binding of metal ion to these RNA structural motifs under physiological conditions. These studies will combine both thermal stability and binding studies to achieve a better understanding of the nature of RNA-metal interactions.