In Situ Hybridization Flow Cytometry Cell Cycle Kinetics

原位杂交流式细胞仪细胞周期动力学

• 批准号: 9709486
• 负责人: Pao Chau
• 金额: $ 19.99万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9709486&HistoricalAwards=false
• 关键词: Situ; Hybridization; Flow; Cytometry; Cell

9709486 Chau The aim of this project is to develop advanced techniques in the study of cell cycle kinetics. This involves: (1) the development of fluorescent in situ hybridization flow cytometry (FISH-FC) using oligonuleotide probes such that one can measure specific messenger RNA of the light of heavy chains of immunoglobulin (2) the measurement of cell cycle kinetic parameters from asynchronous serum-free or protein- free cultures using FISH-FC (DNA and intracellulr immunoglobulin distributions are also to be measured simultaneously with multi-straining); ad (3) the development of a population cell cycle age model which can describe protein production and simulate flow cytometry bivariate distributions. Results of this project should lead to a better understanding of the appropriate cell biology and kinetic phenomena of monoclonal antibody production, especially under serum-free and protein-free conditions. ***

9709486周本项目的目的是开发先进的技术在细胞周期动力学的研究。 这包括：（1）开发了使用寡核苷酸探针的荧光原位杂交流式细胞术（FISH-FC），使得可以测量免疫球蛋白轻链或重链的特异性信使RNA（2）使用FISH-FC测量来自异步无血清或无蛋白培养物的细胞周期动力学参数（DNA和细胞内免疫球蛋白分布也将在多次应变的同时测量）;（3）建立了一个能描述蛋白质产生和模拟流式细胞仪双变量分布的群体细胞周期年龄模型。 该项目的结果应导致更好地了解单克隆抗体生产的适当细胞生物学和动力学现象，特别是在无血清和无蛋白质的条件下。 ***