A Hollow Fiber Bioreactor for the Production of Monoclonal Antibodies

用径向流中空纤维哺乳动物细胞培养系统连续培养非锚定依赖的小鼠杂交瘤。目前正在对持续生产单抗进行研究。杂交瘤细胞系PM-81和AML-2-23用于大规模培养和生产单克隆抗体的研究。该项目还评估了原位膜分离抗体的可行性，并调查了培养和抗体生产的长期稳定性。分析得到了数学模型的补充，这些模型结合了流体力学、膜运输和培养系统的瞬变行为。

A radial flow hollow fiber mammalian cell culture system is to be used for continuous culture of non-anchorage dependent murine hybridoma. Continuous production of monoclonal antibodies is being investigated. Hybridoma cell lines PM-81 and AML-2-23 are used for mass culture and monoclonal antibodies production studies. The project also evaluates the feasibility of ;itin situ;ro membrane separation of antibodies, and investigates the long-term stability of the culture and antibodies production. Analysis is complemented by mathematical models which incorporate hydrodynamics, membrane transport, and transient behavior of culture systems.