A Hollow Fiber Bioreactor for the Production of Monoclonal Antibodies

用于生产单克隆抗体的中空纤维生物反应器

• 批准号: 8512318
• 负责人: Pao Chau
• 金额: $ 6万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-10-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8512318&HistoricalAwards=false
• 关键词: Hollow; Fiber; Bioreactor; Production; Monoclonal

A radial flow hollow fiber mammalian cell culture system is to be used for continuous culture of non-anchorage dependent murine hybridoma. Continuous production of monoclonal antibodies is being investigated. Hybridoma cell lines PM-81 and AML-2-23 are used for mass culture and monoclonal antibodies production studies. The project also evaluates the feasibility of ;itin situ;ro membrane separation of antibodies, and investigates the long-term stability of the culture and antibodies production. Analysis is complemented by mathematical models which incorporate hydrodynamics, membrane transport, and transient behavior of culture systems.

将使用径向流中空纤维哺乳动物细胞培养系统 用于非锚定依赖性鼠杂交瘤的连续培养。 正在研究单克隆抗体的连续生产。 将杂交瘤细胞系PM-81和AML-2-23用于大量培养， 单克隆抗体生产研究。 该项目还评估了 抗体原位反渗透膜分离的可行性， 研究培养物和抗体的长期稳定性 生产 数学模型补充了分析， 结合流体力学、膜运输和瞬态行为 的文化系统。