Homologous chromosome pairing in Schizosaccharomyces pombe

粟酒裂殖酵母同源染色体配对

• 批准号: 116489490
• 负责人: Professorin Iva Marija Tolic, Ph.D.
• 金额: --
• 依托单位: Institut Ruder Boskovic (RBI)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/116489490?language=en
• 关键词: Homologous; chromosome; pairing; Schizosaccharomyces; pombe

Pairing of homologous chromosomes is a crucial step in meiosis. A central question is how homo-logues find each other. The fission yeast Schizosaccharomyces pombe is an excellent model system to study pairing, because of an extensive chromosome movement in the process of "horsetail" nuclear oscillations and a lack of post-pairing synaptic structures. The role of chromosome movement in chromosome pairing has been established by genetic approaches, yet the mechanism of pairing is poorly understood. The aim of this project is to uncover how chromosomes undergo pairing, by taking cell biological and biophysical approaches. We will observe specific loci on homologous chromosomes by two-color imaging, using lac and tet operator/repressor recognition systems. The labeled loci will be tracked with automated tracking software. Nuclear movements will be perturbed at different time points during nuclear oscillations, in order to elucidate the impact of chromosome stretching and relaxation on pairing. Our work is expected to reveal the biophysical mechanisms underlying chromosome pairing in S. pombe.

同源染色体配对是减数分裂的关键步骤。一个核心问题是同语者如何找到彼此。裂殖酵母裂殖酵母是一个很好的研究配对的模型系统，因为在“马尾”核振荡过程中广泛的染色体运动和缺乏配对后突触结构。染色体运动在染色体配对中的作用已经通过遗传学方法建立，但配对的机制知之甚少。该项目的目的是通过细胞生物学和生物物理学方法揭示染色体如何进行配对。我们将通过双色成像，使用lac和泰特操纵子/阻遏子识别系统观察同源染色体上的特定位点。将使用自动跟踪软件跟踪标记的基因座。在核振荡过程中，为了阐明染色体拉伸和弛豫对配对的影响，在不同的时间点将扰动核运动。我们的工作有望揭示S染色体配对的生物物理机制。粟酒