Fabrication of a High Throughput Flow Imaging Cytometer for Analysis of Plankton Populations

小行星9711168 毕格罗海洋科学实验室将进行仪器开发， 一个项目，以建立一个成像流式细胞仪，可以计数和分类浮游生物在 尺寸范围为3至1200微米，并为生物学提供了新工具 海洋学和沿海监测。这个系统将使研究人员能够快速计算， 自动对中型和大型浮游生物进行成像和测量大小， 实验室密集型方法。商用和实验室流式细胞仪可用于 海洋研究涵盖了0.3至100微米的细胞大小。这不是 因为在100至1000微米范围内有许多微生物 存在于海洋和内陆沃茨。此外，这些系统不拍摄 测量的细胞，留给用户有限的信息。 提出的仪器是基于一个工作的原型，并采用细胞 成像、数字图像处理以及荧光和散射光监测， 分析上述尺寸范围内的海洋颗粒。拟设系统可 提高原型的灵敏度和荧光及散射光触发 它已经证明了便携式荧光触发成像的有用性 系统该仪器计划用于几项研究，包括大型浮游植物 放牧和毒性，有害藻类监测，沿海监测和分布 海洋球菌

9711168 Sieracki The Bigelow Laboratory for Ocean Sciences will undertake an instrument development project to construct an imaging flow cytometer that can enumerate and classify plankton in the size range of 3 to 1200 micrometers and provide a new tool for biological oceanography and coastal monitoring. This system will allow researchers to rapidly count, image and size medium and large plankton automatically, instead of with conventional, laboratory intensive methods. Commercial and laboratory flow cytometers available for marine research cover the range of cell sizes from 0.3 to 100 micrometers. This is not sufficient since there are many microbial organisms in the 100 to 1000 micrometer range present in marine and inland waters. Furthermore, these systems do not take images of the measured cells, leaving the user with limited information. The instrument proposed is based on a working prototype and employs cell imaging, digital image processing and fluorescence and scattered light monitoring to analyze marine particles in the size range mentioned above. The proposed system will improve the sensitivity and fluorescence and scattered light triggering of the prototype which has already proven the usefulness of a portable fluorescence triggered imaging system. The instrument is planned for use in several studies, including large phytoplankton grazing and toxicity, harmful algae monitoring, coastal monitoring and the distribution of marine coccoliths.