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Fabrication of a High Throughput Flow Imaging Cytometer for Analysis of Plankton Populations

用于浮游生物种群分析的高通量流式成像细胞仪的制造

基本信息

批准号：
9711168
负责人：
Michael Sieracki
金额：
$ 34.03万
依托单位：
Bigelow Laboratory for Ocean Sciences
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-15 至 2000-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9711168&HistoricalAwards=false
关键词：
Fabrication Throughput Flow Imaging Cytometer

项目摘要

9711168 Sieracki The Bigelow Laboratory for Ocean Sciences will undertake an instrument development project to construct an imaging flow cytometer that can enumerate and classify plankton in the size range of 3 to 1200 micrometers and provide a new tool for biological oceanography and coastal monitoring. This system will allow researchers to rapidly count, image and size medium and large plankton automatically, instead of with conventional, laboratory intensive methods. Commercial and laboratory flow cytometers available for marine research cover the range of cell sizes from 0.3 to 100 micrometers. This is not sufficient since there are many microbial organisms in the 100 to 1000 micrometer range present in marine and inland waters. Furthermore, these systems do not take images of the measured cells, leaving the user with limited information. The instrument proposed is based on a working prototype and employs cell imaging, digital image processing and fluorescence and scattered light monitoring to analyze marine particles in the size range mentioned above. The proposed system will improve the sensitivity and fluorescence and scattered light triggering of the prototype which has already proven the usefulness of a portable fluorescence triggered imaging system. The instrument is planned for use in several studies, including large phytoplankton grazing and toxicity, harmful algae monitoring, coastal monitoring and the distribution of marine coccoliths.

9711168 Sieracki 毕格罗海洋科学实验室将承担一项仪器开发项目，建造一台成像流式细胞仪，可以对尺寸范围为3至1200微米的浮游生物进行计数和分类，为生物海洋学和海岸监测提供新工具。该系统将使研究人员能够自动快速对中型和大型浮游生物进行计数、成像和尺寸测量，而不是使用传统的实验室密集型方法。可用于海洋研究的商业和实验室流式细胞仪涵盖 0.3 至 100 微米的细胞尺寸范围。这还不够，因为海洋和内陆水域中存在许多 100 至 1000 微米范围的微生物。此外，这些系统不会拍摄被测细胞的图像，给用户留下的信息有限。所提出的仪器基于工作原型，并采用细胞成像、数字图像处理以及荧光和散射光监测来分析上述尺寸范围内的海洋颗粒。所提出的系统将提高原型的灵敏度以及荧光和散射光触发，这已经证明了便携式荧光触发成像系统的有用性。该仪器计划用于多项研究，包括大型浮游植物的放牧和毒性、有害藻类监测、海岸监测和海洋石珊瑚的分布。