FSML: A New Generation Flow Cytometer with High-Speed Sorting for Aquatic Sciences Research and Training

FSML：用于水生科学研究和培训的具有高速分选功能的新一代流式细胞仪

• 批准号: 9907566
• 负责人: Michael Sieracki
• 金额: $ 13.51万
• 依托单位: Bigelow Laboratory for Ocean Sciences
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9907566&HistoricalAwards=false
• 关键词: FSML; New; Generation; Flow; Cytometer

This project supports the acquisition of a new generation flow cytometer with high-speed cell Sorting to replace the 17 year old Coulter EPICS V cell sorting instrument at Bigelow Laboratory'sJ.J. MacIsaac Facility for Flow and Imaging Cytometry. This shared-use instrument will be available to aquatic science researchers for a wide range of research applications, and for training undergraduate students, and researchers in aquatic sciences. In addition to improved reliability, the upgraded instrument will provide several major advantages over the present one including: 1) increased sensitivity for detection of submicrometer particles, 2) the ability to sort larger particles(30-60 M), dual-laser excitation capability, and 4) improved computer hardware and software for easier instrument setup, control, and data analysis capabilities. Acquisition of this instrument is an integral part of Bigelow Laboratory's scientific plan to build upon our capabilities in microbiology, phycology, single-cell analysis and species-based ecological approaches to questions in biological oceanography. The MacIsaac Facility is a unique center for advanced research, development and training in automated methods for studying particles in naturals waters. In the past 6 years the Facility has served over 25 investigators from 20 different institutions in the U.S. and internationally. It is a center for innovation, having spawned several new research and development directions, including the recent invention of an imaging-in-flow instrument for microplankton (20-200 gm) analysis. As part of the shared-use MacIsaac Facility the new instrument wil enhance several areas of new research opportunities for researchers in acquit sciences. These include the development of new single-cell fluorescence methods for studying bacteria, phytoplankton and microzooplankton physiology, the physiology of harmful algae species, ecological studies of plankton populations, the application of "phylogenetic stains" for aquatic plankton, the chemical and optical properties of aquatic particles, and the molecular genetics of subpopulations. The new generation flow cytometer with high speed sorting will significantly enhance the continued education and training activities in this technology. Bigelow Laboratory courses and workshops attract graduate students and postdoctoral researchers from around the world. A modem instrument for education and training is essential for maintaining this leadership role. The instrument wil be integrated into the J. J. MacIsaac Facility, which has actively served the international acquatic science

该项目支持收购新一代具有高速细胞分选功能的流式细胞仪，以取代Bigelow实验室的J.J. MacIsaac流式细胞仪和成像细胞仪中使用了17年的Coulter EPICS V细胞分选仪。 这一共享使用的仪器将提供给水产科学研究人员，用于广泛的研究应用，并用于培训本科生和水产科学研究人员。 除了提高可靠性外，升级后的仪器将提供几个主要优点，包括：1）增加检测亚微米颗粒的灵敏度，2）分选较大颗粒（30-60 M）的能力，双激光激发能力，以及4）改进的计算机硬件和软件，使仪器设置，控制和数据分析能力更容易。 购买这台仪器是毕格罗实验室科学计划的一个组成部分，该计划旨在加强我们在微生物学、藻类学、单细胞分析和以物种为基础的生态方法来解决生物海洋学问题方面的能力。 MacIsaac设施是一个独特的中心，用于研究自然沃茨中颗粒的自动化方法的高级研究，开发和培训。 在过去的6年里，该机构为来自美国和国际上20个不同机构的25名调查人员提供了服务。 它是一个创新中心，催生了几个新的研究和发展方向，包括最近发明的流动成像仪器，用于微型浮游生物（20-200克）分析。 作为共享使用的MacIsaac设施的一部分，新仪器将为成就科学的研究人员提供新的研究机会。 其中包括开发新的单细胞荧光方法，用于研究细菌、浮游植物和微型浮游动物的生理学、有害藻类的生理学、浮游生物种群的生态学研究、水生浮游生物“系统发生染色剂”的应用、水生颗粒的化学和光学特性以及亚种群的分子遗传学。 具有高速分选功能的新一代流式细胞仪将大大加强这一技术的继续教育和培训活动。 毕格罗实验室的课程和研讨会吸引了来自世界各地的研究生和博士后研究人员。 现代化的教育和培训手段对保持这种领导作用至关重要。 该仪器将被集成到J. J. MacIsaac设施中，该设施积极服务于国际水产科学