5 laser flow cytometer for the detection of 20 parameters with high throughput sampling unit

根据该提案，Benjamin富兰克林流式细胞术机构（BFFC）申请了一台能够进行多参数分析（18个荧光参数、2个散射检测器、5个激光器和一个高通量采样单元）的额外流式细胞术分析仪。目前，Charité - Universitätsmedizin柏林的Benjamin富兰克林校园（CBF）正在进行重大扩建，并通过招募新的实验室负责人及其团队进行科学重建。新的流式细胞仪是迫切需要的分析和功能表征（包括细胞周期分析和Ca 2+信号）的罕见和/或复杂的细胞群体的最高水平，无论是在基础和临床研究。BFFC已经运行了一个类似的分析仪，但机器已经在最大容量下运行，导致长时间的等待和项目的延误。Charité没有其他流式细胞仪设施提供具有5个激光器的类似分析仪。多参数流式细胞术是基本上所有生物医学科学中的“最先进”技术。特别地，临床样品的分析需要快速访问这样的分析仪，以便保证及时分析这些珍贵的样品。功能强大的高通量分析仪有助于快速分析多个样品，并建立新颖而强大的体外模型（例如，单细胞分化测定、类器官技术）。由于CBF最近招募了新的团队，因此迫切需要一种新的流式细胞术分析仪来科普对多参数流式细胞术日益增长的需求。

With this proposal, the Benjamin Franklin Flow Cytometry Facility (BFFC) applies for an additional flow cytometry analyzer capable of multiparameter analysis (18 fluorescence parameters, 2 scatter detectors, 5 lasers and a high throughput sampling unit). Currently, the Campus Benjamin Franklin (CBF) of Charité - Universitätsmedizin Berlin undergoes a significant expansion and is being scientifically re-invigorated by the recruitment of new laboratory heads and their groups. The new flow cytometer is urgently needed for the analysis and functional characterization (including cell cycle analysis and Ca2+ signaling) of rare and/or complex cell populations on the highest possible level, both in basic and clinical research. BFFC already operates a similar analyzer but the machine already runs at maximal capacity leading to long waiting times and delays in projects. No other flow cytometry facility at Charité offers a comparable analyzer with 5 lasers. Multiparameter flow cytometry is a “state-of-the-art” technology in basically all biomedical sciences. In particular, the analysis of clinical samples requires rapid access to such an analyzer, in order to guarantee timely analysis of these precious samples. The powerful high-throughput analyzer facilitates rapid analysis of multiple samples and the establishment of novel and powerful in vitro models (e.g., single cell differentiation assays, organoid technology). Due to the recent recruitment of new groups at CBF a new flow cytometry analyzer is urgently needed to cope with the increasing demand for multi-parameter flow cytometry.