5-laser flow cytometer with high-throughput unit

具有高通量单元的 5 激光流式细胞仪

• 批准号: 442249343
• 金额: --
• 依托单位: Georg-August-Universität Göttingen
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/442249343?language=en
• 关键词: laser; flow; cytometer; throughput; unit

We apply for a 5-laser flow cytometer with high-throughput platform for the flow cytometry core facility Cell Sorting of the University Medical Center Göttingen (UMG), University of Göttingen. The device will allow cell biology analysis at advanced technical level in the flow cytometry unit, which is in place since 2006. The high multiparametric capacity based on the laser equipment of the new instrument enables the differentiated dissection of the cellular components in tumors and inflammatory tissues, as well as the detection of ectopic expressed fluorescent marker of the mRFPs (monomeric red fluorescent proteins) after genetic transduction. The proposed instrument is designed to measure small sample volumes from small animal studies, and it is suited for automated large scale screening approaches with on a high throughput module. Infrastructural integration in the UMG core facility setting warrants the efficient and flexible use of the instrument by a broad group of specialized research teams.

我们为哥廷根大学医学中心（UMG）的流式细胞术核心设施细胞分选申请了一台带有高通量平台的5激光流式细胞仪。该设备将允许在自2006年以来设置的流式细胞仪单元中进行先进技术水平的细胞生物学分析。新仪器基于激光设备的高多参数能力能够区分肿瘤和炎症组织中的细胞成分，以及检测基因转导后mRFPs（单体红色荧光蛋白）的异位表达荧光标记。所提出的仪器被设计用于测量来自小动物研究的小样品体积，并且它适合于具有高通量模块的自动化大规模筛选方法。UMG核心设施设置中的结构集成保证了广泛的专业研究团队对仪器的有效和灵活使用。