Mechanism of Adsorption, Film Formation, and Conformational Stability of Proteins at Liquid Interfaces

蛋白质在液体界面的吸附、成膜和构象稳定性机制

• 批准号: 9712197
• 负责人: Srinivasan Damodaran
• 金额: $ 17.87万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9712197&HistoricalAwards=false
• 关键词: Mechanism; Adsorption; Film; Formation; Conformational

9712197 Damodaran Protein adsorption and film formation at various interfaces play important roles in many biological and technological processes. Despite the enormous importance of this field, fundamental understanding of these processes, especially adsorption at the lipid-water interface, is still elusive. Thus, the main objectives of this project are: (1) to develop experimental approaches to study the kinetics and thermodynamics of the air-water interface; (2) to elucidate the relationship between the molecular flexibility and the equation of state of proteins at air-water and oil-water interfaces; (3) to elucidate the molecular factors affecting competitive adsorption of proteins from binary and ternary protein solutions to the oil-water interface; and (4) to investigate the thermodynamic compatibility and two dimensional phase separation behavior of proteins in a binary protein film at air-water and oil-water interfaces. The outcome from this project is to generate the basic information needed to understand various biological, chemical, and technological processes which involve protein/enzyme adsorption at air-liquid and liquid-liquid interfaces. ***

蛋白质在各种界面上的吸附和成膜在许多生物和工艺过程中起着重要作用。尽管这一领域具有巨大的重要性，但对这些过程的基本理解，特别是在脂水界面的吸附，仍然是难以捉摸的。因此，本项目的主要目标是：(1)发展研究空气-水界面动力学和热力学的实验方法；(2)阐明了蛋白质在空气-水和油水界面的状态方程与分子柔韧性的关系；(3)阐明影响二元和三元蛋白质溶液对油水界面竞争吸附的分子因素；(4)研究了二元蛋白质膜中蛋白质在空气-水和油水界面的热力学相容性和二维相分离行为。这个项目的结果是产生理解各种生物、化学和技术过程所需的基本信息，这些过程涉及在空气-液体和液-液体界面上的蛋白质/酶吸附。＊＊＊