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Analysis of Two Genes Expressed in Prepatterns in the Shoot Apex of Arabidopsis

拟南芥茎尖预模式表达的两个基因分析

基本信息

批准号：
9712927
负责人：
Patricia Springer
金额：
$ 3.85万
依托单位：
University of California-Riverside
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1997
资助国家：
美国
起止时间：
1997-06-01 至 1999-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9712927&HistoricalAwards=false
关键词：
Analysis Two Genes Expressed Prepatterns

项目摘要

Springer 9712927 A transposon tagging system has been established using Ds transposable elements containing reporter genes. A collection of lines, each containing a Ds element inserted randomly in the Arabidopsis genome, has been generated. The Ds element carries a reporter gene, allowing the expression pattern of nearby genes to be monitored via the reporter gene. These lines have been screened for reporter gene expression in the shoot apex of Arabidopsis seedlings. Several genes have been identified that are specifically expressed in discrete domains within the shoot apex. These include genes expressed specifically in the shoot apical meristem, and genes expressed in domains at the base of each leaf primordium. Two lines (GT185 and GT522) have been chosen for further characterization. These lines both exhibit expression of the reporter gene in a group of cells subtending each organ (rosette leaf, cauline leaf, flower) and organ primordium. The expression pattern suggests that the genes controlling the reporter gene may be involved in the establishment of a boundary between the shoot apical meristem and organ primordia. As a first step in the characterization of the genes controlling reporter gene expression in these two lines, the reporter gene expression pattern will be carefully analyzed at different developmental stages. The genes controlling reporter gene expression will be cloned by amplification of DNA flanking the Ds insertion. The genes will be molecularly characterized by sequencing and in-situ hybridization. The relationship between GT185, GT522 and two genes that have previously been shown to play a role in meristem function will be examined.

利用含有报告基因的Ds转座子元件建立了转座子标记系统。在拟南芥基因组中随机插入了一组含有Ds元素的细胞系。Ds元件携带一个报告基因，允许通过报告基因监测附近基因的表达模式。对这些品系进行了拟南芥幼苗茎尖报告基因表达的筛选。已经确定了在茎尖内的离散域特异性表达的几个基因。这些基因包括在茎尖分生组织中特异性表达的基因，以及在每片叶原基基部表达的基因。选择两个品系（GT185和GT522）进行进一步表征。这些细胞系都在每个器官（莲座叶、茎叶、花）和器官原基的一组细胞中表达报告基因。这种表达模式表明，控制报告基因的基因可能参与了茎尖分生组织和器官原基之间边界的建立。作为鉴定这两个品系中控制报告基因表达的基因的第一步，我们将仔细分析报告基因在不同发育阶段的表达模式。控制报告基因表达的基因将通过扩增Ds插入的DNA来克隆。这些基因将通过测序和原位杂交进行分子表征。GT185、GT522与两个先前被证明在分生组织功能中起作用的基因之间的关系将被研究。