The Developmental Role of Lateral Organ Boundaries and Related Genes

侧器官边界及相关基因的发育作用

• 批准号: 0318822
• 负责人: Patricia Springer
• 金额: $ 36万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0318822&HistoricalAwards=false
• 关键词: Developmental; Role; Lateral; Organ; Boundaries

0318822SpringerLeaves and other lateral organs initiate from the flanks of the shoot apical meristem (SAM), and forming lateral organs develop axes of asymmetry in response to positional cues from the SAM. The LATERAL ORGAN BOUNDARIES (LOB) gene is expressed in a band of cells between the SAM and initiating lateral organs. LOB expression is regulated both by genes that are important for SAM formation, and genes that are important for leaf development, suggesting that LOB may be involved in signaling between the SAM and organ primordia. LOB encodes a novel protein that is unique to plants and defines the conserved LOB domain. Forty-three Arabidopsis genes encode proteins that contain a LOB domain. Among these, ASYMMETRIC LEAVES2 (AS2), LBD10, LBD25, and LBD36 are most closely related to LOB. Of the LOB-domain genes thus far analyzed, only AS2 results in a visible phenotype when mutated. These observations suggest that the members of the LOB-domain gene family exhibit functional redundancy. Ectopic expression of AS2 results in the formation of lateral organs that have abnormal abaxial-adaxial polarity, implicating AS2, and perhaps closely related genes, in the establishment adaxial cell fates. This research focuses on understanding the developmental role of LOB and related genes. LOB and closely related genes will be characterized by genetic analyses. Plants that carry single or multiple mutant combinations will be identified to uncover the developmental roles each gene plays. The role of AS2 in polarity determination will be investigated. LOB cis-acting regulatory sequences will be characterized. Proteins that interact with LOB will be identified. The long-term goals of this research are to understand the mechanisms of lateral organ formation from the shoot apical meristem, and in particular what role LOB-domain genes play in this process. The project will provide training for undergraduate and graduate students and a postdoctoral associate in a high minority-enrollment institution.

0318822春叶和其他侧生器官从茎顶端分生组织（SAM）的侧面开始，并且形成侧生器官响应于来自SAM的位置线索而发展不对称的轴。 外侧器官边界（LOB）基因在SAM和起始外侧器官之间的细胞带中表达。 LOB表达受SAM形成的重要基因和叶发育的重要基因调控，表明LOB可能参与SAM和器官原基之间的信号传导。 LOB编码一种植物特有的新蛋白，并定义了保守的LOB结构域。 43个拟南芥基因编码含有LOB结构域的蛋白质。其中，AS 2、LBD 10、LBD 25和LBD 36与LOB的关系最为密切。 在迄今为止分析的LOB结构域基因中，只有AS 2在突变时导致可见的表型。 这些观察结果表明，LOB结构域基因家族的成员表现出功能冗余。 AS 2的异位表达导致侧器官的形成，这些侧器官具有异常的远轴-近轴极性，这暗示AS 2以及可能密切相关的基因在建立近轴细胞命运中。 本研究的重点是了解LOB和相关基因的发育作用。 LOB和密切相关的基因将通过遗传分析来表征。 将鉴定携带单个或多个突变体组合的植物，以揭示每个基因所起的发育作用。 将研究AS 2在极性测定中的作用。 将表征LOB顺式作用调控序列。 将鉴定与LOB相互作用的蛋白质。 本研究的长期目标是了解茎尖分生组织侧生器官形成的机制，特别是LOB结构域基因在这一过程中发挥的作用。该项目将在少数民族入学率高的机构为本科生和研究生以及博士后助理提供培训。