CAREER: Genetics of Pattern Formation in the Arabidopsis Shoot Apex

职业：拟南芥芽尖模式形成的遗传学

• 批准号: 9875371
• 负责人: Patricia Springer
• 金额: $ 39.25万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9875371&HistoricalAwards=false
• 关键词: CAREER; Genetics; Pattern; Formation; Arabidopsis

The shoot apical meristem (SAM) is the ultimate source of all above groundtissues of the plant. The SAM gives rise to leaves, stems, flowers, andaxillary buds, and is responsible for the pattern of the shoot. Despitethe crucial role of the shoot apical meristem in plant development, almostnothing is known about the mechanisms by which organs are produced from theflanks of this structure. Using a novel mutagenesis system, enhancer trap(ET) and gene trap (GT) transposant lines that exhibit GUS reporter geneexpression in discrete domains in the SAM or surrounding cells have beenidentified. Two transposant lines show GUS expression specific to the SAM. The genes controlling GUS expression in these lines could play a role inSAM identity and/or function. Eight transposant lines have been identifiedthat show GUS expression in discrete, novel domains surrounding the SAM.These expression patterns do not appear to reflect anatomical ormorphological features, but define new domains based on gene expression.The genes controlling GUS expression in these lines may function to defineboundaries during organ initiation. These lines were identified based onGUS expression patterns, and the genes controlling that expression are ofunknown function. A series of analyses will be performed in order to beginto understand the role that these genes play in meristem function andorganogenesis. The genes will be cloned and sequenced, and a detailedcharacterization of their expression patterns will be performed. In orderto identify genetic relationships between genes that function in themeristem, GUS expression in our gene trap and enhancer trap lines will beexamined in backgrounds containing mutations that affect meristem function,and in transgenic plants that ectopically express selected meristem genes. The educational component of this proposal is geared towards trainingundergraduate and graduate students and postdoctoral researchers in modernmethods in Arabidopsis research. Arabidopsis thaliana is a model organismfor studies in plant biology. The many available Arabidopsis resources areof use to all plant biology researchers. In order for plant biologyresearchers to be able to take full advantage of the Arabidopsis resourcesthat are available, it is important that they be familiar with the standardmethods used in Arabidopsis research. To this end, an intensive laboratorycourse will be developed, that will teach standard Arabidopsis methods.Materials from the PI's research program will be used for the laboratories. In addition, the PI will serve as a mentor to graduate students andpostdoctoral researchers who will carry out the research component of thisproposal.

茎尖分生组织（SAM）是植物所有地上组织的最终来源。SAM产生了叶、茎、花和腋芽，并负责芽的模式。尽管茎尖分生组织在植物发育中起着至关重要的作用，但关于该结构的侧翼产生器官的机制几乎一无所知。利用一种新的诱变系统，增强子陷阱（ET）和基因陷阱（GT）转座系在SAM或周围细胞的离散区域中表现出GUS报告基因表达。两条转座细胞系显示了SAM特有的GUS表达。在这些细胞系中控制GUS表达的基因可能在inSAM的身份和/或功能上起作用。已经鉴定出8条转座线，在SAM周围的离散的新结构域中显示GUS表达。这些表达模式似乎并不反映解剖或形态特征，但根据基因表达定义了新的结构域。在这些细胞系中，控制GUS表达的基因可能在器官形成过程中起着确定边界的作用。这些系是根据onGUS表达模式鉴定的，而控制该表达的基因功能未知。为了开始了解这些基因在分生组织功能和器官发生中的作用，将进行一系列的分析。这些基因将被克隆和测序，并对其表达模式进行详细的表征。为了确定在分生组织中起作用的基因之间的遗传关系，我们的基因陷阱和增强子陷阱系将在包含影响分生组织功能的突变的背景下以及在异位表达所选分生组织基因的转基因植物中检测GUS表达。本提案的教育部分旨在培养拟南芥研究的现代方法的本科生、研究生和博士后研究人员。拟南芥（Arabidopsis thaliana）是植物生物学研究的模式生物。许多可用的拟南芥资源可供所有植物生物学研究人员使用。为了使植物生物学研究人员能够充分利用现有的拟南芥资源，熟悉拟南芥研究中使用的标准方法是很重要的。为此，将开发一门密集的实验室课程，教授标准的拟南芥方法。来自PI研究项目的材料将用于实验室。此外，PI将作为研究生和博士后研究人员的导师，他们将执行本提案的研究部分。