Epigenetic control of meiotic recombination in Drosophila

果蝇减数分裂重组的表观遗传控制

• 批准号: 117267854
• 负责人: Professor Dr. Gunter Reuter (†)
• 金额: --
• 依托单位: Abteilung Entwicklungsgenetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/117267854?language=en
• 关键词: Epigenetic; control; meiotic; recombination; Drosophila

The main aim of our studies consists in resolving epigenetic processes, which control meiotic recombination in Drosophila. Analysis of histone modifications at SCs revealed abundant H3K9me3 and H3K27 methylation marks suggesting a preferential heterochromatin like state of chromatin at synaptonemal complexes. Abundant binding of proteins controlling gene silencing could also be demonstrated. Mutant analysis suggests a central role of the histone methyltransferases SETDB1 and E(Z) in control of chromatin organization at SCs. An impact of chromatin organization on recombination frequency and crossover distribution is indicated by first data of high-resolution crossover mapping in selected genomic regions. In our further studies we focus on the analysis of epigenetic factors which control epigenetic indexing and higher order structure of chromatin at synaptonemal complexes. By high-resolution crossover analysis the impact of these factors on crossover frequency and distribution will be studied.

我们研究的主要目的在于解决控制果蝇减数分裂重组的表观遗传过程。 SC 组蛋白修饰分析揭示了丰富的 H3K9me3 和 H3K27 甲基化标记，表明联会复合体染色质存在优先异染色质样状态。还可以证明控制基因沉默的蛋白质的丰富结合。突变分析表明组蛋白甲基转移酶 SETDB1 和 E(Z) 在控制 SC 染色质组织中发挥核心作用。选定基因组区域中高分辨率交叉作图的第一个数据表明了染色质组织对重组频率和交叉分布的影响。在我们进一步的研究中，我们重点分析控制表观遗传索引和联会复合体染色质高阶结构的表观遗传因素。通过高分辨率交叉分析，将研究这些因素对交叉频率和分布的影响。