Biodegradation of Subsurface Pollutants by Chlorate- Respiring Microorganisms

9714575洛根之前，关于这一主题的探索性研究是由国家科学基金会探索性研究小额拨款项下的首席调查员进行的，编号94-14423。这项更新奖的研究目的是继续确定在其代谢过程中使用能够利用氯酸盐作为电子受体的微生物来净化土壤和含水层的可行性。研究人员计划通过使用呼吸抑制剂来剖析电子传输链，对比研究基于氯酸盐的微生物呼吸和分子氧途径。为确定土壤和含水层污染物的生物降解性，本研究中要调查的土壤和含水层污染物包括甲苯、对二甲苯、乙苯、萘、四氯化碳、氯苯酚、氯苯甲酸酯、苯、戊烷和苯酚。根据拟议研究结果设想的去污过程将涉及将氯酸盐和氯酸盐呼吸有机体同时注入受污染的土壤或含水层。该项目的结果有望为其在工程设计中的潜在应用提供依据，该系统在阻止或禁止使用大气来源的氧气的情况下，使用氯酸钠作为呼吸氧源来净化土壤和地下水含水层。与其他潜在的化学电子受体，如硝酸盐和硫酸盐，其副产物是潜在的地下水污染物，生化氯酸盐利用的唯一副产品是氯离子。***

9714575 Logan Prior exploratory research on this topic was conducted by the Principal Investigator under NSF Small Grant for Exploratory Research Number 94-14423. The objective of research on this renewal award is to continue determination of the feasibility of using microorganisms capable of using chlorate as an electron acceptor in their metabolic processes for decontamination of soil and aquifers. The investigators plan on studying chlorate-based microbial respiration in contrast with molecular oxygen pathways by use of respiratory inhibitors to dissect the electron transport chain. Soil and aquifer contaminants to be investigated in this research for determination of their biodegradability by chlorate-respiring microorganisms include toluene, p-xylene, ethylbenzene, naphthalene, carbon tetrachloride, chlorophenol, chlorobenzoate, benzene, pentane and phenol. The contemplated decontamination process based on results of the proposed research would involve simultaneous injection of chlorates and chlorate-respiring organisms into the contaminated soil or aquifer. Results of this project are expected to provide the basis for its potential application in engineering design of a system to decontaminate soil and groundwater aquifers by use of sodium chlorate as a source of respiratory oxygen under conditions that prevent or inhibit use of oxygen from atmospheric sources. In contrast to other potential chemical electron acceptors such as nitrates and sulfates, byproducts of which are potential groundwater pollutants, the only byproduct of biochemical chlorate utilization is the chloride ion. ***