Molecular Level Analysis of Macromolecule-Surface Interactions in Bacterial Adhesion

细菌粘附中大分子-表面相互作用的分子水平分析

• 批准号: 0089156
• 负责人: Bruce Logan
• 金额: $ 255万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0089156&HistoricalAwards=false
• 关键词: Molecular; Level; Analysis; Macromolecule; Surface

The Special Projects Office in the Chemistry Division supports this Collaborative Research Activities in Environmental Molecular Science (CRAEMS) Award to Penn State University. This award supports Bruce Logan, Darrel Velegol, Jon Chorover and James Kubicki to study macromolecule-surface interactions in bacterial adhesion in natural and engineered aquatic systems at the molecular level. In addition, the award supports Menachem Elimelech at Yale University through a subaward to study theoretical and experimental aspects of bacterial cell motion and adhesion near interfaces. The underlying hypothesis is that cell-surface interactions are dominated by the interaction of cell-surface polymers (exopolymers), and natural organic matter, such as humic and fulvic acids, with the surface. Atomic Force Spectroscopy, FTIR, electro-hydrodynamic techniques, and molecular modeling will be employed to probe polymer size, strength, and conformation as a function of solution chemistry, surface charge, and surface composition. The goal is improved methods and models that describe interactions between particles and surfaces that are irregularly shaped at atomic levels in the presence of bonded and soluble macromolecules.This research is designed to improve our understanding of bacterial adhesion in natural systems and in engineered environments. It has important implications for restoration of contaminated groundwater, water treatment, and wastewater reclamation, which will become increasingly important as availability of fresh water becomes a global environmental and human health concern. Outreach programs educate students of the local school system in environmental problems and approaches.

化学部的特别项目办公室支持向宾夕法尼亚州立大学颁发环境分子科学合作研究活动奖。该奖项支持Bruce Logan、Darrel Velegol、Jon Chorover和James Kubicki在分子水平上研究天然和工程水生系统中细菌黏附中的大分子-表面相互作用。此外，该奖项还通过一个子奖支持耶鲁大学的梅纳赫姆·埃米勒，该奖项旨在研究细菌细胞运动和界面附近黏附的理论和实验方面。基本的假设是，细胞-表面相互作用是由细胞-表面聚合物(胞外聚合物)以及腐殖酸和富里酸等天然有机物与表面的相互作用所主导的。原子力光谱、FTIR、电流体动力学技术和分子模拟将被用来探测聚合物的大小、强度和构象作为溶液化学、表面电荷和表面组成的函数。目标是改进描述粒子和表面之间的相互作用的方法和模型，这些粒子和表面在原子水平上是不规则形状的，存在键合的和可溶的大分子。这项研究旨在提高我们对自然系统和工程环境中细菌黏附的理解。它对修复受污染的地下水、水处理和废水回收具有重要意义，随着淡水的可获得性成为全球环境和人类健康问题，这些问题将变得越来越重要。外展项目对当地学校系统的学生进行环境问题和方法方面的教育。